Yeast engineered translucent cell wall to provide its endosymbiont cyanobacteria with light.

Archives of Microbiology
Hoda EbrahimiParastoo Saniee

Abstract

In this study, relationship between translucent property of yeast cell wall and occurrence of cyanobacteria inside the yeast vacuole was examined. Microscopic observations on fruit yeast Candida tropicalis showed occurrence of bacterium-like bodies inside the yeast vacuole. Appearance of vacuoles as distinct cavities indicated the perfect harvesting of light by the yeast's cell wall. Transmission electron microscopy observation showed electron-dense outer and electron-lucent inner layers in yeast cell wall. Cyanobacteria-specific 16S rRNA gene was amplified from total DNA of yeast. Cultivation of yeast in distilled water led to excision of intracellular bacteria which grew on cyanobacteria-specific medium. Examination of wet mount and Gram-stained preparations of excised bacteria showed typical bead-like trichomes. Amplification of cyanobacteria-specific genes, 16S rRNA, cnfR and dxcf, confirmed bacterial identity as Leptolyngbya boryana. These results showed that translucent cell wall of yeast has been engineered through evolution for receiving light for vital activities of cyanobacteria.

References

Jan 1, 1991·Critical Reviews in Biotechnology·S Bielecki, E Galas
May 1, 1971·Scientific American·E S Barghoorn
Apr 17, 1997·Nature·J HughesT Börner
Aug 1, 1997·Applied and Environmental Microbiology·U NübelG Muyzer
Jul 31, 1998·Journal of Bacteriology·P N Lipke, R Ovalle
Jul 8, 2000·Trends in Ecology & Evolution·N A Moran, J J Wernegreen
Sep 14, 2001·Molecular Microbiology·C W Mullineaux
May 10, 2002·Nature·Eric GiraudAndré Verméglio
Apr 23, 2003·Research in Microbiology·Ilana Berman-FrankPaul Falkowski
Jul 23, 2003·Environmental Microbiology·Meike Hoffmeister, William Martin
Aug 15, 2003·Nature·Pete Vukusic, J Roy Sambles
Oct 7, 2003·Annual Review of Microbiology·James A Imlay
Oct 26, 2005·Current Biology : CB·Anne BlumensteinReinhard Fischer
Jul 1, 1985·Applied and Environmental Microbiology·P A Murray, S H Zinder
Dec 22, 2005·Biotechnology Letters·Hai-Rong Cheng, Ning Jiang
Mar 23, 2007·Molecular Microbiology·Alfredo Herrera-Estrella, Benjamin A Horwitz
Apr 6, 2007·BMC Evolutionary Biology·Christoph KneipUwe-G Maier
Feb 9, 2008·Annual Review of Plant Biology·Gabyong Bae, Giltsu Choi
Oct 25, 2008·Nature·Birger RasmussenMatt R Kilburn
May 14, 2009·The Journal of General and Applied Microbiology·Takayuki Yoshida, Toshio Sakamoto
Jun 4, 2010·PloS One·John M PisciottaIlia V Baskakov
Jun 11, 2010·Annual Review of Microbiology·Julio Rodriguez-RomeroReinhard Fischer
May 25, 2012·Nature·Rachel S EdgarAkhilesh B Reddy
Apr 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ryoma TsujimotoYuichi Fujita
Aug 15, 2015·Journal of Pineal Research·Lucien C ManchesterRussel J Reiter
May 3, 2018·Nutrition Reviews·Mark LucockEmma Beckett
Jan 27, 2019·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Farideh SiavoshiShadi Kolahdoozan
Dec 17, 2019·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Samira HeydariAmir Houshang Sharifi

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Datasets Mentioned

BETA
MF629803.1
MH688846
NIES-2135
AP018203.1

Methods Mentioned

BETA
light microscopy
fluorescence microscopy
PCR

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