Yeast-expressed recombinant protein of the receptor-binding domain in SARS-CoV spike protein with deglycosylated forms as a SARS vaccine candidate

Human Vaccines & Immunotherapeutics
Wen-Hsiang ChenShibo Jiang

Abstract

Development of vaccines for preventing a future pandemic of severe acute respiratory syndrome (SARS) caused by SARS coronavirus (SARS-CoV) and for biodefense preparedness is urgently needed. Our previous studies have shown that a candidate SARS vaccine antigen consisting of the receptor-binding domain (RBD) of SARS-CoV spike protein can induce potent neutralizing antibody responses and protection against SARS-CoV challenge in vaccinated animals. To optimize expression conditions for scale-up production of the RBD vaccine candidate, we hypothesized that this could be potentially achieved by removing glycosylation sites in the RBD protein. In this study, we constructed two RBD protein variants: 1) RBD193-WT (193-aa, residues 318-510) and its deglycosylated forms (RBD193-N1, RBD193-N2, RBD193-N3); 2) RBD219-WT (219-aa, residues 318-536) and its deglycosylated forms (RBD219-N1, RBD219-N2, and RBD219-N3). All constructs were expressed as recombinant proteins in yeast. The purified recombinant proteins of these constructs were compared for their antigenicity, functionality and immunogenicity in mice using alum as the adjuvant. We found that RBD219-N1 exhibited high expression yield, and maintained its antigenicity and functionality. ...Continue Reading

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Citations

Mar 18, 2016·Expert Review of Vaccines·Lanying DuShibo Jiang
Jul 30, 2020·PLoS Neglected Tropical Diseases·Peter J Hotez, Maria Elena Bottazzi
Jun 28, 2020·Journal of Virology·John P Moore, P J Klasse
Mar 29, 2020·Current Tropical Medicine Reports·Wen-Hsiang ChenMaria Elena Bottazzi
Aug 15, 2020·Frontiers in Immunology·Lea Skak Filtenborg FrederiksenAneesh Thakur
Jun 1, 2018·Future Virology·To Sing Fung, Ding Xiang Liu
Apr 9, 2020·Frontiers in Microbiology·Ning WangLanying Du
Jan 1, 2016·Current Tropical Medicine Reports·Hai Yen LeeUlrich Strych
Oct 18, 2020·Nature Reviews. Microbiology·Shan SuShibo Jiang
Jan 12, 2021·The Journal of Biological Chemistry·Sameer Kumar MalladiRaghavan Varadarajan
Jan 10, 2021·Advanced Drug Delivery Reviews·Jeroen PolletUlrich Strych
Jan 12, 2021·Frontiers in Immunology·Jing ZhaoQiwei Zhang
Mar 19, 2021·Biochimica Et Biophysica Acta. General Subjects·Wen-Hsiang ChenMaria Elena Bottazzi
Apr 26, 2021·International Immunopharmacology·Seyede Atefe HosseiniReza Kazemi Oskuee
May 7, 2021·IUCrJ·Marek GrabowskiWladek Minor
May 8, 2021·Applied Microbiology and Biotechnology·Jungsoon LeeMaria Elena Bottazzi
Jun 5, 2021·Applied Microbiology and Biotechnology·R Sahaya GlingstonShirisha Nagotu
Jul 3, 2021·Biochemistry·Joel D AllenMax Crispin
Jul 30, 2021·Frontiers in Immunology·Daniel Martínez-FloresLuis Vaca
Sep 7, 2021·Frontiers in Bioengineering and Biotechnology·Johannes RuhnauUdo Reichl
Sep 9, 2021·Proceedings of the National Academy of Sciences of the United States of America·Neil C DalvieJ Christopher Love

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