Yeast Expression Systems: Overview and Recent Advances

Molecular Biotechnology
Roghayyeh BaghbanMaryam Aria

Abstract

Yeasts are outstanding hosts for the production of functional recombinant proteins with industrial or medical applications. Great attention has been emerged on yeast due to the inherent advantages and new developments in this host cell. For the production of each specific product, the most appropriate expression system should be identified and optimized both on the genetic and fermentation levels, considering the features of the host, vector and expression strategies. Currently, several new systems are commercially available; some of them are private and need licensing. The potential for secretory expression of heterologous proteins in yeast proposed this system as a candidate for the production of complex eukaryotic proteins. The common yeast expression hosts used for recombinant proteins' expression include Saccharomyces cerevisiae, Pichia pastoris, Hansenula polymorpha, Yarrowia lipolytica, Arxula adeninivorans, Kluyveromyces lactis, and Schizosaccharomyces pombe. This review is dedicated to discuss on significant characteristics of the most common methylotrophic and non-methylotrophic yeast expression systems with an emphasis on their advantages and new developments.

References

Nov 1, 1986·Molecular and Cellular Biology·M J CharronC A Michels
May 11, 1987·Nucleic Acids Research·J F TschoppT R Gingeras
Dec 1, 1985·Molecular and Cellular Biology·J M CreggK R Madden
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H KojoA Sugino
Dec 1, 2000·Molecular Biotechnology·J M CreggD R Higgins
Sep 11, 2002·Progress in Nucleic Acid Research and Molecular Biology·Heinz-Peter NasheuerKlaus Weisshart
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Roy M WilliamsRochelle E Esposito
Mar 5, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M W Cook, H V Thygesen
Apr 2, 2003·Journal of Biotechnology·Mehmedalija JahicSven-Olof Enfors
Apr 19, 2003·FEMS Yeast Research·Adelheid DegelmannGerd Gellissen
Apr 19, 2003·FEMS Yeast Research·Jean-Marc NicaudClaude Gaillardin
Apr 6, 2004·Journal of Biotechnology·Catherine MadzakJean-Marie Beckerich
Oct 14, 2004·Applied Microbiology and Biotechnology·F R Schmidt
Nov 27, 2004·Journal of Molecular Recognition : JMR·Rachel Daly, Milton T W Hearn
Dec 4, 2004·Biotechnology Progress·Oliver TrentmannFrank Hoffmann
May 13, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Patricia MuñozTeresa Peláez
Oct 19, 2005·Biotechnology Letters·Yueyong LiuBingsheng Qiu
Feb 9, 2007·FEMS Yeast Research·Gábor PéterDénes Dlauchy
Feb 13, 2007·Applied Microbiology and Biotechnology·Bärbel Hahn-HägerdalMarie F Gorwa-Grauslund
Sep 19, 2007·Methods in Enzymology·Stefan HohmannBodil Nordlander
Nov 21, 2007·Applied Biochemistry and Biotechnology·Pingzuo LiV Renugopalakrishnan
Nov 28, 2007·Biochimica Et Biophysica Acta·H Reynaldo López-Mirabal, Jakob R Winther
Jan 30, 2008·Biotechnology Journal·Leonor Miller-FlemingTiago F Outeiro

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Citations

Jun 10, 2020·Fungal biology and biotechnology·Hung Hiep HuynhJun-Ichi Maruyama
May 20, 2020·Applied Microbiology and Biotechnology·Martina BorčinováKarin Kovar
May 2, 2020·Expert Review of Clinical Pharmacology·Kamila Botelho Sampaio de OliveiraSimoni Campos Dias
Nov 9, 2019·Extremophiles : Life Under Extreme Conditions·Ryota KurashikiHirokazu Suzuki
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Nagesh K Tripathi, Ambuj Shrivastava
Jul 17, 2020·Frontiers in Bioengineering and Biotechnology·Miguel Angel Nieto-TaypeFrancisco Valero
Feb 9, 2021·Bioactive Materials·Shuo-Fu YuanHal S Alper
Feb 18, 2021·FEMS Yeast Research·Dmitry Mamaev, Renata Zvyagilskaya
Apr 8, 2021·Protein Expression and Purification·Claudia DurantiAnnarosa Arcangeli
May 1, 2021·Microorganisms·Hiroya YurimotoYasuyoshi Sakai
May 4, 2021·Applied Microbiology and Biotechnology·Radin Shafierul Radin YahayaSuriana Sabri
May 3, 2021·Biotechnology Letters·Renan Eugênio Araujo PiraineFábio Pereira Leivas Leite
Jun 12, 2021·Heliyon·Zhiger AkishevBekbolat Khassenov
Aug 17, 2021·Frontiers in Bioengineering and Biotechnology·Chun-Qiang LiPei-Ji Zhao
Jun 26, 2020·Journal of Agricultural and Food Chemistry·Hong SunHao Song
Dec 16, 2021·Critical Reviews in Food Science and Nutrition·Zhi-Xiang XiangJin-Song Shi
Jan 8, 2022·ACS Synthetic Biology·Zhiqian WangHoujin Zhang
Oct 7, 2021·Angewandte Chemie·Cameron C HannaRichard J Payne

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