PMID: 74Nov 20, 1975

Yeast glutathione reductase. Studies of the kinetics and stability of the enzyme as a function of pH and salt concentration

Biochimica Et Biophysica Acta
G Moroff, K G Brandt

Abstract

1. The pH dependencies of the apparent Michaelis constant for oxidized glutathione and the apparent turnover number of yeast glutathione reductase (EC 1.6.4.2) have been determined at a fixed concentration of 0.1 mM NADPH in the range pH 4.5--8.0. Between pH 5.5 and 7.6, both of these parameters are relatively constant. The principal effect of low pH on the kinetics of the enzyme-catalyzed reaction is the observation of a pH-dependent substrate inhibition by oxidized glutathione at pH less than or equal 7, which is shown to correlate with the binding of oxidized glutathione to the oxidized form of the enzyme. 2. The catalytic activity of yeast glutathione reductase at pH 5.5 is affected by the sodium acetate buffer concentration. The stability of the oxidized and reduced forms of the enzyme at pH 5.5 and 25 degrees C in the absence of bovine serum albumin was studied as a function of sodium acetate concentration. The results show that activation of the catalytic activity of the enzyme at low sodium acetate concentration correlates with an effect of sodium acetate on a reduced form of the enzyme. In contrast, inhibition of the catalytic activity of the enzyme at high sodium acetate concentration correlates with an effect of sodi...Continue Reading

References

Aug 21, 1973·Biochemical and Biophysical Research Communications·B Mannervik
Mar 14, 1973·Journal of Chromatography·J J Harding
Apr 8, 2011·Chemistry : a European Journal·Guo-Liang WangGuo-Xin Jin

Citations

Jan 17, 2014·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Kavita AroraArvind K Srivastava
Jan 1, 1993·The International Journal of Biochemistry·C García-AlfonsoJ López-Barea
Apr 1, 1995·The International Journal of Biochemistry & Cell Biology·M J McCallum, J Barrett
Jun 5, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Luciano A CardosoMarcelo Hermes-Lima
Sep 2, 1985·European Journal of Biochemistry·M C PintoJ López-Barea
Aug 1, 1976·European Journal of Biochemistry·D J Worthington, M A Rosemeyer
Mar 10, 1981·The Journal of Comparative Neurology·B H Choi

Related Concepts

Acetic Acids
Drug Stability
Enzyme Activation
Reduced Glutathione
Glutathione Reductase
Hydrogen-Ion Concentration
Osmolality
Oxidation-Reduction
Saccharomyces cerevisiae

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