PMID: 1253Nov 15, 1975

Yeast hexokinase A. Succinylation and properties of the active subunit

European Journal of Biochemistry
A RossiJ Pudles

Abstract

Yeast hexokinase A (ATP:D-hexose 6-phosphotransferase, EC2.7.1.1) dissociates into its subunits upon reaction with succinic anhydride. The chemically modified subunits could be isolated in a catalytically active form. The Km values found for ATP and for glucose were of the some order as those found for the native enzyme. Of the 37 amino groups present per enzyme subunit, 2-3 of these groups might be located in the proximity of the region of subunit interactions. The 50% loss of the initial activity, which follows the succinylation of these more reactive amino groups, does not seem to be due to the modification of a residue on the enzyme active site or to a change of the tertiary structure of the protein. This 50%loss of the enzyme activity may be related to the dissociation of the dimer into monomers. Both native enzyme and the succinylated subunits have the same H-dependent denaturation rate profiles in response to 2 M urea. Moreover, the apparent pK of the group involved in the transition from a more stable conformation of the protein in the acid range to a less stable one at alkaline pH seems to be similar to the pK of the group implicated in the transition between the protonated inactive form of the enzyme and an active dep...Continue Reading

References

Jul 13, 1972·European Journal of Biochemistry·J S Easterby, M A Rosemeyer
Nov 15, 1973·European Journal of Biochemistry·M GrouselleJ Pudles
Jul 1, 1974·Archives of Biochemistry and Biophysics·A C Chou, J E Wilson
Jan 1, 1971·Preparative Biochemistry·Y M RustumE A Barnard
Jun 1, 1971·The Biochemical Journal·J P Shill, K E Neet
Jun 29, 1971·Biochimica Et Biophysica Acta·A Z Budzynski, G E Means
Mar 12, 1968·Biochemical and Biophysical Research Communications·T R Hopkins, J D Spikes
Jun 1, 1968·Biochemistry·N R LazarusE A Barnard
Sep 26, 1966·Archives of Biochemistry and Biophysics·S Anderson, G Weber
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN

Citations

Feb 17, 2015·Molecular and Biochemical Parasitology·Héctor AcostaJuan Luis Concepción
Dec 1, 1978·Archives of Biochemistry and Biophysics·F Womack, S P Colowick
Aug 15, 1988·Analytical Biochemistry·R N Puri, R Roskoski

Related Concepts

Acylation
Adenosine Triphosphate, Chromium Ammonium Salt
Citrates
Enzyme Activation
Calcium Glycerophosphate
Hexokinase 2
Hydrogen-Ion Concentration
Magnesium
Malates
Orthophosphate

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