PMID: 6989607Apr 1, 1980Paper

Yeast mannosyl transferases requiring dolichyl phosphate and dolichyl phosphate mannose as substrate. Partial purification and characterization of the solubilized enzyme

European Journal of Biochemistry
P BabczinskiW Tanner

Abstract

The first mannosyl unit of manno-oligosaccharides of fungal mannoproteins is transferred in a dolichyl-phosphate-dependent reaction sequence to serine/threonine residues of the protein. The two membrane-bound enzymes catalyzing this transfer in the yeast Saccharomyces cerevisiae have been solubilized by detergents. The enzyme transferring mannose from guanosine diphosphate mannose to dolichyl phosphate has been purified 18-fold when based on membrane protein and 140-fold when based on total cell protein. The enzyme transferring mannose from dolichyl phosphate mannose to protein has been purified 48-fold and 380-fold, respectively. A HCl-treated cell-wall mannoprotein from yeast served as acceptor protein for the second enzyme. The solubilized enzyme catalyzing the formation of dolichyl diphosphate mannose has a Km for guanosine diphosphate mannose of 7 x 10(-6) M and is saturated with about 0.15 mM yeast dolichyl phosphate. The metal requirement, pH-optima, and the detergent concentration necessary for optimal activity have been determined for both solubilized enzymes.

References

Apr 23, 1979·Biochimica Et Biophysica Acta·A J Parodi, L F Leloir
Jan 1, 1976·Annual Review of Biochemistry·C J Waechter, W J Lennarz
Mar 7, 1977·Biochemical and Biophysical Research Communications·A Heifetz, A D Elbein
Apr 11, 1977·Biochemical and Biophysical Research Communications·W Thomas ForseeJohn S Schutzbach
Aug 1, 1973·European Journal of Biochemistry·P Jung, W Tanner
Oct 1, 1973·Biochemical and Biophysical Research Communications·P Babczinski, W Tanner
May 20, 1974·Biochimica Et Biophysica Acta·Ludwig Lehle, W Tanner
Jul 1, 1974·European Journal of Biochemistry·C B SharmaW Tanner
Sep 1, 1968·The Biochemical Journal·R Sentandreu, D H Northcote
Apr 10, 1969·Biochemical and Biophysical Research Communications·W Tanner

Citations

Oct 15, 1998·International Journal for Parasitology·L Vargas-RodríguezE López-Romero
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A Haselbeck, W Tanner
Nov 15, 1985·European Journal of Biochemistry·J W Jensen, John S Schutzbach
Apr 5, 2008·Biochimica Et Biophysica Acta·Yusuke Maeda, Taroh Kinoshita
Apr 1, 1980·European Journal of Biochemistry·G PalamarczykW Tanner
May 1, 1981·European Journal of Biochemistry·C B SharmaW Tanner
Dec 24, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·W TannerLudwig Lehle
Jul 27, 2017·Nature Communications·Rosaria GandiniChristina Divne

Related Concepts

Chromatography
Dolichol Monophosphate Mannose
Dolichol Monophosphates
Hexosyltransferase
Hydrogen-Ion Concentration
Magnesium
Manganese
Mannosyltransferases
Vigilon
Polyisoprenyl Phosphate Sugars

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Archaeal RNA Polymerase

Archaeal RNA polymerases are most similar to eukaryotic RNA polymerase II but require the support of only two archaeal general transcription factors, TBP (TATA-box binding protein) and TFB (archaeal homologue of the eukaryotic general transcription factor TFIIB) to initiate basal transcription. Here is the latest research on archaeal RNA polymerases.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.