Yeast Mitochondrial Transcription Factor Mtf1 Determines the Precision of Promoter-Directed Initiation of RNA Polymerase Rpo41

PloS One
Xu YangY Whitney Yin

Abstract

Despite their clear T7-bacteriophage origin, mitochondrial RNA polymerases have evolved to require transcription factors. All mitochondrial polymerases contain an extra N-terminal domain that has no counterpart in the self-proficient phage enzyme, which is therefore hypothesized to interact with transcription factors. We studied a series of N-terminal deletion mutants of yeast mitochondrial RNA polymerase, Rpo41, and have found that the N-terminal region does not abolish the effects of Mtf1; rather it contributes directly to enzyme catalysis. Mtf1 can rescue the defective Rpo41 enzymes resulted from N-terminal domain deletions. Although Rpo41 appears to have retained all promoter recognition elements found in T7 RNAP, the elements are not independently functional, and Mtf1 is necessary and sufficient for holoenzyme promoter-directed transcription activity.

References

Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T K BiswasG S Getz
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·G M CheethamT A Steitz
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y Wang, G S Shadel
Dec 22, 1999·Science·G M Cheetham, T A Steitz
May 2, 2001·Cell Biochemistry and Biophysics·A J KalH F Tabak
Sep 22, 2001·Protein Science : a Publication of the Protein Society·F D SchubotB C Wang
Jun 18, 2002·Nature Genetics·Maria FalkenbergClaes M Gustafsson
Nov 8, 2002·Nature·Tahir H TahirovShigeyuki Yokoyama
Dec 24, 2002·Nature Genetics·Bonnie L Seidel-RogolGerald S Shadel
Mar 25, 2004·Protein Expression and Purification·Michio MatsunagaJudith A Jaehning
Sep 3, 2004·The Journal of Biological Chemistry·Michio Matsunaga, Judith A Jaehning
Aug 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sjoerd WanrooijMaria Falkenberg
Jan 1, 2009·The Journal of Biological Chemistry·Guo-Qing TangSmita S Patel
Mar 25, 2009·The Journal of Biological Chemistry·Dhananjaya NayakRui Sousa
Nov 19, 2009·The Journal of Biological Chemistry·Maria SavkinaMichael Anikin
Dec 17, 2009·The Journal of Biological Chemistry·Swaroopa Paratkar, Smita S Patel
Feb 5, 2010·Molecular Cell·Javier Miralles FustéMaria Falkenberg
Apr 2, 2011·The Journal of Biological Chemistry·Swaroopa ParatkarSmita S Patel

❮ Previous
Next ❯

Methods Mentioned

BETA
gel filtration
PCR

Software Mentioned

Quantity One

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.