Yeast mutation thought to arrest mRNA transport markedly increases the length of the 3' poly(A) on polyadenylated RNA.

Journal of Molecular Biology
P W Piper, J L Aamand

Abstract

In Saccharomyces cerevisiae the function of the RN A1 gene is believed to be required for the transport of newly synthesized mRNA from the nucleus to the cytoplasm. Nuclear poly(A)+ RNAs accumulate and cytoplasmic mRNAs decay after the temperature-sensitive (ts) rna1.1 mutant is shifted from 25 degrees C to 37 degrees C. In this study the 3' poly(A) upon poly(A)+ RNA synthesized after expression of rna1.1 was shown to be appreciably longer than the poly(A) normally present on yeast cytoplasmic mRNA. This increased poly(A) length is due to rna1.1, since it was found only in this mutant after a 25 degrees C to 37 degrees C heat shock, not an intragenic non-ts revertant of rna1.1, wild-type (RN A1+) cells or a RN A1+, rna2.1 mutant subjected to equivalent heat shocks. It may be an indication that the normal shortening of the poly(A) on mRNAs does not occur in the nucleus, but happens only with transport to the cytoplasm. Alterations in the mean size of poly(A) may be a relatively simple marker for mRNA transport defects.

References

Feb 26, 1979·Molecular & General Genetics : MGG·H Koch, J D Friesen
Feb 26, 1979·Molecular & General Genetics : MGG·L L Chia, C McLaughlin
Nov 11, 1986·Nucleic Acids Research·T C SantiagoA J Brown
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Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B W Baer, R D Kornberg

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Citations

Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·H M TragliaA K Hopper
Apr 1, 1995·Molecular Biology of the Cell·R SchneiterA M Tartakoff
Aug 5, 2003·Molecular and Cellular Biology·Biswadip DasFred Sherman
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·K S MysoreS B Gelvin
Dec 1, 1990·Current Opinion in Cell Biology·A Sachs

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