Yeast pre-mRNA splicing requires a pair of U1 snRNP-associated tetratricopeptide repeat proteins.

Molecular and Cellular Biology
M R McLean, B C Rymond

Abstract

The U1 snRNP functions to nucleate spliceosome assembly on newly transcribed pre-mRNA. Saccharomyces cerevisiae is unusual among eukaryotes in the greatly extended length of its U1 snRNA and the apparent increased polypeptide complexity of the corresponding U1 snRNP. In this paper, we report the identification of a novel U1 snRNP protein, Prp42p, with unexpected properties. Prp42p was identified by its surprising structural similarity to the essential U1 snRNP protein, Prp39p. Both Prp39p and Prp42p possess multiple copies of a variant tetratricopeptide repeat, an element implicated in a wide range of protein assembly events. Yeast strains depleted of Prp42p by transcriptional repression of a GAL1::PRP42 fusion gene arrest for splicing prior to pre-mRNA 5' splice site cleavage. Prp42p was not observed in a recent biochemical analysis of purified U1 snRNPs from S. cerevisiae (28). Nevertheless, antibodies directed against an epitope-tagged version of Prp42p specifically precipitate U1 snRNA from yeast extracts. Furthermore, Prp42p is required for U1 snRNP biogenesis, because yeast strains depleted of Prp42p formed incomplete U1 snRNPs that failed to produce stable complexes with pre-mRNA in vitro. The evidence shows that Prp39p ...Continue Reading

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Citations

Jul 26, 2000·The Journal of Cell Biology·S M Mount, H K Salz
Aug 7, 2002·Biochemical and Biophysical Research Communications·Sophie Raisin-Tani, Pierre Léopold
Dec 24, 1998·Nucleic Acids Research·R L SeipeltB C Rymond
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Jan 2, 2003·The Journal of Biological Chemistry·Qiang WangBrian C Rymond
Oct 1, 2003·Molecular and Cellular Biology·Qiang Wang, Brian C Rymond
Aug 5, 2003·Molecular and Cellular Biology·Kimberly M KotovicKarla M Neugebauer
May 9, 2001·Biochimica Et Biophysica Acta·C H LaiW Lin
Jun 27, 2002·Biochimica Et Biophysica Acta·Seyung ChungBrian C Rymond

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