Yeast protein kinase Ptk2 localizes at the plasma membrane and phosphorylates in vitro the C-terminal peptide of the H+-ATPase

Biochimica Et Biophysica Acta
Pilar ErasoF Portillo

Abstract

Glucose triggers posttranslational modifications that increase the activity of the Saccharomyces cerevisiae plasma membrane H+-ATPase (Pma1). Glucose activation of yeast H+-ATPase results from the change in two kinetic parameters: an increase in the affinity of the enzyme for ATP, depending on Ser899, and an increase in the Vmax involving Thr912. Our previous studies suggested that Ptk2 mediates the Ser899-dependent part of the activation. In this study we find that Ptk2 localized to the plasma membrane in a Triton X-100 insoluble fraction. In vitro phosphorylation assays using a recombinant GST-fusion protein comprising 30 C-terminal amino acids of Pma1 suggest that Ser899 is phosphorylated by Ptk2. Furthermore, we show that the Ptk2 carboxyl terminus is essential for glucose-dependent Pma1 activation and for the phosphorylation of Ser899.

References

Jan 1, 1991·Methods in Enzymology·J R PringleB K Haarer
Nov 22, 1995·Biochemical and Biophysical Research Communications·Y KakinumaK Igarashi
Mar 15, 1993·European Journal of Biochemistry·R SerranoE W Weiler
Dec 13, 1996·The Journal of Biological Chemistry·E EstradaM Ghislain
Jan 1, 1997·Trends in Biochemical Sciences·T Hunter, G D Plowman
Oct 19, 1999·Molecular and Cellular Biology·J E Hirschman, D D Jenness
Feb 29, 2000·Biochimica Et Biophysica Acta·F Portillo
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Oct 23, 2003·The Journal of Biological Chemistry·Ge-Fei ZengClifford L Slayman
Jul 2, 2005·Molecular Cell·Frédérique DuboulozClaudio De Virgilio

❮ Previous
Next ❯

Citations

Jun 1, 2010·The Journal of Biological Chemistry·Theodore T Diakov, Patricia M Kane
Apr 19, 2012·The Journal of Biological Chemistry·Hyemin KimKyle W Cunningham
May 27, 2008·The Journal of Biological Chemistry·Gloria A Martínez-Muñoz, Patricia Kane
Oct 3, 2009·Genes & Development·Jessica C S Brown, Susan Lindquist
Mar 7, 2013·Genetics·Martha S Cyert, Caroline C Philpott
Mar 5, 2013·Journal of Bioscience and Bioengineering·Hideharu TakashitaKazuhisa Ono
Sep 24, 2009·Fungal Genetics and Biology : FG & B·Roger R Lew, Vitaliy Kapishon
Mar 13, 2014·The FEBS Journal·Femke I C MensonidesM Joost Teixeira de Mattos
Jun 19, 2008·FEMS Microbiology Reviews·Juana M Gancedo
May 29, 2015·FEMS Yeast Research·María J MazónFrancisco Portillo
Apr 23, 2016·Journal of Bioscience and Bioengineering·Minetaka SugiyamaSatoshi Harashima
Nov 1, 2016·FEMS Yeast Research·Yu Chen, Jens Nielsen
Mar 20, 2020·PLoS Genetics·Jessica M TumoloJason A MacGurn
Nov 30, 2016·The Journal of Biological Chemistry·Cecilia PrimoLynne Yenush
Mar 13, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yadira G Ruiz-GranadosJosé G Sampedro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.