PMID: 9159392May 1, 1997Paper

Yeast Rad55 and Rad57 proteins form a heterodimer that functions with replication protein A to promote DNA strand exchange by Rad51 recombinase

Genes & Development
P Sung

Abstract

Saccharomyces cerevisiae RAD51, RAD55, and RAD57 genes, required for genetic recombination and DNA double-strand-break repair, encode proteins homologous to one another and to the Escherichia coli RecA protein. Rad51 protein catalyzes the DNA strand-exchange reaction with a dependence on ATP and on the heterotrimeric single-strand DNA (ssDNA) binding factor replication protein A (RPA). By several independent criteria, RAD55- and RAD57-encoded products are shown here to exist as a stable heterodimer, with a dissociation constant of <2 x 10(-10) M. In strand exchange, the reaction proceeds efficiently if RPA is incorporated after nucleation of Rad51 onto ssDNA, but if RPA is present during the nucleation phase, as is likely the case in vivo, the amount of strand-exchange products becomes relatively insignificant. Inclusion of the Rad55-Rad57 heterodimer with Rad51 and RPA results in a marked stimulation of strand exchange, providing evidence for a role of the Rad55-Rad57 heterodimer in overcoming the inhibitory effect of RPA.

References

Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P SungS Prakash
Jan 1, 1987·Annual Review of Biochemistry·M M Cox, I R Lehman
May 1, 1983·Cell·J W SzostakF W Stahl
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·S L HaysP Berg
Sep 1, 1995·Molecular and Cellular Biology·R D Johnson, L S Symington
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K UmezuR D Kolodner
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S N GuzderS Prakash
Nov 8, 1996·The Journal of Biological Chemistry·P Sung, S A Stratton

❮ Previous
Next ❯

Citations

Apr 12, 2013·Archives of Virology·Geetika SuyalNirupam R Choudhury
Apr 30, 2013·DNA Repair·William J GrahamCynthia A Haseltine
May 29, 2004·Mutation Research·Andrej Dudás, Miroslav Chovanec
Apr 15, 2004·Mutation Research·Yael Aylon, Martin Kupiec
Aug 1, 2000·Mutation Research·P SungS Van Komen
Aug 1, 2000·Mutation Research·J C Game
May 30, 2001·Mutation Research·L H Thompson, D Schild
Aug 17, 2001·Mutation Research·A PastinkP H Lohman
Nov 13, 2002·Mutation Research·Larry H Thompson, David Schild
May 14, 1999·Trends in Genetics : TIG·J Thacker
Dec 6, 2000·Mutation Research· van den Bosch MA Pastink
Aug 23, 2001·Mutation Research·M L Dronkert, R Kanaar
Oct 20, 1999·Mutation Research·A Shinohara, T Ogawa
Dec 23, 1998·Trends in Cell Biology·R KanaarD C van Gent
Aug 11, 1998·Trends in Biochemical Sciences·P Baumann, S C West
Jul 3, 1999·Trends in Biochemical Sciences·J E Haber
Sep 29, 1999·Trends in Biochemical Sciences·H T Beernink, S W Morrical
Apr 30, 2003·Molecular Cell·Judith Henry-MowattKeith W Caldecott
Oct 13, 1999·Mutation Research·A Pastink, P H Lohman
Jan 2, 2008·Cell Research·Xuan Li, Wolf-Dietrich Heyer
Aug 24, 2006·Nature Reviews. Molecular Cell Biology·Patrick Sung, Hannah Klein
Aug 22, 2006·Nature Structural & Molecular Biology·Nami HarutaHiroshi Iwasaki
Mar 10, 2009·The Journal of Biological Chemistry·Susan R FerrariDouglas K Bishop
Aug 16, 2008·The Journal of Biological Chemistry·Iben PlateUffe H Mortensen
Nov 7, 2008·The Journal of Biological Chemistry·Akiko Sakai, Michael M Cox
Oct 19, 2010·Carcinogenesis·Kumar SomyajitGanesh Nagaraju
Feb 28, 1998·Nucleic Acids Research·O V KovalenkoC M Radding

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.