Yeast-secreted bovine herpesvirus type 1 glycoprotein D has authentic conformational structure and immunogenicity

Vaccine
X ZhuG J Letchworth

Abstract

Bovine herpesvirus-1 (BHV-1) glycoprotein D (gD), an envelope glycoprotein, engenders mucosal and systemic immunity protecting cattle from viral infection. Production of gD with authentic immunogenicity is required for a subunit vaccine. We placed the truncated BHV-1 gD gene, lacking its putative transmembrane and cytoplasmic domains, under the control of the methanol-inducible AOX1 promoter in the yeast Pichia pastoris. Truncated BHV-1 gD (tgD) was efficiently secreted into the culture medium as a 68 kDa protein using either the yeast alpha prepro or native BHV-1 gD signal sequences. The yeast-secreted tgD had N-linked glycosylation and appears to have authentic conformational structure and immunogenicity based on the following observations A panel of monoclonal antibodies recognizing five neutralizing epitopes reacted with yeast tgD. Sera from yeast tgD-immunized mice immunoprecipitated native BHV-1 gD and neutralized BHV-1 infection in vitro. Yeast tgD competitively blocked all reaction between native gD and monospecific gD polyclonal sera from cattle. Based on these data, yeast-derived BHV-1 tgD is an excellent candidate for a subunit vaccine.

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Citations

Jan 21, 2000·FEMS Microbiology Reviews·J L Cereghino, J M Cregg
Nov 21, 2008·Molecular Immunology·Jianzhong ZhuGeorge K Mutwiri
Nov 2, 2014·Veterinary Research·Luana Alves DummerSylvia van Drunen Littel-van den Hurk
Mar 25, 2014·Vaccine·Luana Alves DummerFábio Pereira Leivas Leite
Mar 26, 1998·Plasmid·M T Dertzbaugh
Jan 23, 2019·FEMS Yeast Research·Ravinder Kumar, Piyush Kumar
Jan 30, 2007·Veterinary Research·Benoît MuylkensEtienne Thiry

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