Yeast surface display for screening combinatorial polypeptide libraries

Nature Biotechnology
E T Boder, K D Wittrup

Abstract

Display on the yeast cell wall is well suited for engineering mammalian cell-surface and secreted proteins (e.g., antibodies, receptors, cytokines) that require endoplasmic reticulum-specific post-translational processing for efficient folding and activity. C-terminal fusion to the Aga2p mating adhesion receptor of Saccharomyces cerevisiae has been used for the selection of scFv antibody fragments with threefold decreased antigen dissociation rate from a randomly mutated library. A eukaryotic host should alleviate expression biases present in bacterially propagated combinatorial libraries. Quantitative flow cytometric analysis enables fine discrimination of kinetic parameters for protein binding to soluble ligands.

References

Aug 5, 1992·Journal of Molecular Biology·R E HawkinsG Winter
Aug 1, 1984·Molecular and Cellular Biology·M Johnston, R W Davis
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·S J DengS A Narang
Mar 1, 1995·Microbiological Reviews·E M Phizicky, S Fields
Jan 1, 1994·Annual Review of Immunology·G WinterH R Hoogenboom
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J A FranciscoG Georgiou
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·H D UlrichP G Schultz
Apr 1, 1996·Trends in Biotechnology·M P SchreuderF M Klis

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Citations

Mar 17, 1999·Journal of Molecular Recognition : JMR·J J VanAntwerp, K D Wittrup
Oct 10, 2001·Angewandte Chemie·Sean V. TaylorDonald Hilvert
Feb 14, 2002·Journal of Cellular Biochemistry. Supplement·D Ma, M Li
Apr 17, 2007·Molecular Biotechnology·Ren RenDongzhi Wei
Jan 22, 2013·Archives of Microbiology·Setareh TavassoliGholamreza Ahmadian
Sep 25, 2008·Applied Microbiology and Biotechnology·Takashi AdachiAkihiko Kondo
Dec 25, 2009·Applied Microbiology and Biotechnology·Guo-Dong SuYing Lin
Aug 3, 2010·Applied Microbiology and Biotechnology·Wen-Shan LiuYun-Jun Yan
Jun 2, 2012·Applied Microbiology and Biotechnology·Tsutomu TanakaAkihiko Kondo
Sep 28, 2010·Biotechnology Letters·Kouichi Kuroda, Mitsuyoshi Ueda
Jul 21, 2010·Molecular Biotechnology·Jeffrey S SwersK Dane Wittrup
May 8, 2013·Biologicals : Journal of the International Association of Biological Standardization·Nur Hidayah Hairul BaharaTheam Soon Lim
Apr 17, 2004·Journal of Immunological Methods·Robert W SiegelMichael J Feldhaus
Apr 22, 2004·Journal of Immunological Methods·Jennifer R CochranK Dane Wittrup
Aug 13, 2013·Trends in Biotechnology·Christine C LeePeter M Tessier
May 24, 2005·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·S SiberilJ-L Teillaud
Nov 1, 2002·Biochemical and Biophysical Research Communications·Daniela UccellettiClaudio Palleschi
Nov 5, 2002·Clinical Biochemistry·Hassan M E Azzazy, W Edward Highsmith
Sep 15, 2000·Journal of Immunological Methods·P S DaughertyG Georgiou
Feb 27, 2001·Journal of Immunological Methods·M D GriffinJ A Bluestone
Dec 31, 2002·Journal of Immunological Methods·Susan E BrophyDavid M Kranz
Dec 5, 1998·Journal of Immunological Methods·B K ChoD M Kranz
Mar 5, 2003·Journal of Molecular Biology·Björn PhilippsRudi Glockshuber
May 22, 2003·Journal of Molecular Biology·Thorsten Lamla, Volker A Erdmann
Dec 19, 2000·Enzyme and Microbial Technology·H Shi, Wen Su W
Aug 1, 2000·Immunology Today·H R Hoogenboom, P Chames
Dec 14, 2002·Trends in Biotechnology·Sang Yup LeeZhaohui Xu
Jun 6, 2003·Trends in Biotechnology·Andrew BradburyPeter Pavlik
Jun 1, 2002·Journal of Biotechnology·Patrik SamuelsonStefan Ståhl
Aug 8, 2002·FEMS Microbiology Reviews·Frans M KlisStanley Brul
Oct 17, 2001·Experimental Hematology·B P Mullaney, M G Pallavicini
Jan 25, 2003·Experimental Hematology·Ruiwu LiuKit S Lam

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