PMID: 7017715Jan 1, 1981Paper

Yeast tRNA precursor mutated at a splice junction is correctly processed in vivo

Proceedings of the National Academy of Sciences of the United States of America
D ColbyC Guthrie

Abstract

Yeast mutants with decreased expression of a tRNATyr gene were obtained by selection for functional inactivation of the tyrosine-inserting ochre suppressor SUP4 and subsequent screening for production of the tRNA gene product in vivo. One mutant with reduced suppressor activity was characterized by a decreased quantity of the suppressor-specific tRNA; a precursor to this tRNA, matured at both 5' and 3' termini but still containing a 14-nucleotide intervening sequence, was present in an amount greater than 7-fold that in the parent. By RNA sequence analysis of the accumulated precursor, we have identified the mutation as an A leads to G transition at the 5' splice junction. Similar analysis of the mature tRNA produced in this mutant demonstrated that the intervening sequence was accurately excised. We conclude that the specific sequence of nucleotides at this splice junction affects the efficiency but not the fidelity of processing.

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Citations

Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S H OrkinR L Hechtman
Sep 10, 1983·Nucleic Acids Research·G J Raymond, J D Johnson
May 18, 2004·Genes & Development·Sujatha KadabaJames Anderson
Dec 31, 2008·FEBS Letters·Hanjiang FuXiaofei Zheng
May 24, 2011·Molecular and Biochemical Parasitology·Sujaan DasGotam K Jarori
Sep 1, 1984·Bioscience Reports·R W Davies
Jan 1, 1984·CRC Critical Reviews in Biochemistry·M P Deutscher
Dec 1, 1988·Molecular and Cellular Biology·S S Wang, A K Hopper
Jul 1, 1986·Molecular and Cellular Biology·M C Strobel, J Abelson

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