PMID: 7527135Nov 11, 1994Paper

Yeast Ty1 retrotransposon: the minus-strand primer binding site and a cis-acting domain of the Ty1 RNA are both important for packaging of primer tRNA inside virus-like particles

Nucleic Acids Research
M WilhelmT Heyman

Abstract

Reverse transcription of the yeast retrotransposon Ty1 is primed by the cytoplasmic initiator methionine tRNA (tRNA(iMet)). The primer tRNA(iMet) is packaged inside virus-like particles (VLPs) and binds to a 10 nucleotides minus-strand primer binding site, the (-)PBS, complementary to its 3' acceptor stem. We have found that three short sequences of the Ty1 RNA (box 1, box 2.1 and box 2.2) located 3' to the (-)PBS are complementary to other regions of the primer tRNA(iMet) (T psi C and DHU stems and loops). Reconstitution of reverse transcription in vitro with T7 transcribed Ty1 RNA species and tRNA(iMet) purified from yeast cells shows that the boxes do not affect the efficiency of reverse transcription. Thus the role of the boxes on packaging of the primer tRNA(iMet) into the VLPs was investigated by analysing the level of tRNA(iMet) packaged into mutant VLPs. Specific nucleotide changes in the (-)PBS or in the boxes that do not change the protein coding sequence but disrupt the complementarity with the primer tRNA(iMet) within the VLPs. We propose that base pairing between the primer tRNA(iMet) and the Ty1 RNA is of major importance for tRNA(iMet) packaging into the VLPs. Moreover the intactness of the boxes is essential for...Continue Reading

References

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Citations

Jan 1, 1995·Biochimie·R MarquetB Ehresmann
Apr 4, 1998·Nucleic Acids Research·C IselR Marquet
Oct 25, 2012·Nucleic Acids Research·Katarzyna J PurzyckaDavid J Garfinkel
Aug 12, 2005·Cytogenetic and Genome Research·F-X WilhelmA Gabriel
Dec 13, 2000·Yeast·E P PlantR T Poulter
Jun 22, 2000·Yeast·J F Roth
Nov 30, 2006·Gene·Eugene A GladyshevIrina R Arkhipova
Aug 27, 2002·The Journal of Biological Chemistry·Valerie GoldschmidtRoland Marquet
Aug 8, 1998·The Journal of Biological Chemistry·C LiangM A Wainberg
Jun 12, 2019·IUBMB Life·Catherine Florentz, Richard Giegé
Jun 25, 2015·Microbiology Spectrum·M Joan CurcioPascale Lesage
Nov 26, 1997·Journal of Bacteriology·G D MatthewsR T Poulter
Oct 29, 1997·Journal of Virology·J Mak, L Kleiman

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