Yellow and Orange Fluorescent Proteins with Tryptophan-based Chromophores

ACS Chemical Biology
Nina G BozhanovaAlexander S Mishin

Abstract

Rapid development of new microscopy techniques exposed the need for genetically encoded fluorescent tags with special properties. Recent works demonstrated the potential of fluorescent proteins with tryptophan-based chromophores. We applied rational design and random mutagenesis to the monomeric red fluorescent protein FusionRed and found two groups of mutants carrying a tryptophan-based chromophore: with yellow (535 nm) or orange (565 nm) emission. On the basis of the properties of proteins, a model synthetic chromophore, and a computational modeling, we concluded that the presence of a ketone-containing chromophore in different isomeric forms can explain the observed yellow and orange phenotypes.

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Citations

Nov 28, 2018·Analytical Chemistry·Xiaoni FangWenwan Zhong

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