Yersinia controls type III effector delivery into host cells by modulating Rho activity

PLoS Pathogens
Edison MejíaGloria I Viboud

Abstract

Yersinia pseudotuberculosis binds to beta1 integrin receptors, and uses the type III secretion proteins YopB and YopD to introduce pores and to translocate Yop effectors directly into host cells. Y. pseudotuberculosis lacking effectors that inhibit Rho GTPases, YopE and YopT, have high pore forming activity. Here, we present evidence that Y. pseudotuberculosis selectively modulates Rho activity to induce cellular changes that control pore formation and effector translocation. Inhibition of actin polymerization decreased pore formation and YopE translocation in HeLa cells infected with Y. pseudotuberculosis. Inactivation of Rho, Rac, and Cdc42 by treatment with Clostridium difficile toxin B inhibited pore formation and YopE translocation in infected HeLa cells. Expression of a dominant negative form of Rac did not reduce the uptake of membrane impermeable dyes in HeLa cells infected with a pore forming strain YopEHJT(-). Similarly, the Rac inhibitor NSC23766 did not decrease pore formation or translocation, although it efficiently hindered Rac-dependent bacterial uptake. In contrast, C. botulinum C3 potently reduced pore formation and translocation, implicating Rho A, B, and/or C in the control of the Yop delivery. An invasin mu...Continue Reading

References

Oct 1, 1992·Infection and Immunity·M Simonet, S Falkow
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·M P SoryG R Cornelis
Jan 20, 1997·The Journal of Experimental Medicine·M WataraiC Sasakawa
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·C J Hueck
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·G R Cornelis
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·M S FassettJ L Strominger
May 15, 2002·International Journal of Medical Microbiology : IJMM·Michelle L ZaharikB Brett Finlay
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Joseph T BarbieriKlaus Aktories
Jul 27, 2002·Journal of Bacteriology·Christophe CarnoyMichel Simonet
Dec 12, 2002·Methods in Enzymology·Gloria I Viboud, James B Bliska
Jan 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Feng ShaoJack E Dixon
May 22, 2003·Advances in Experimental Medicine and Biology·Feng Shao, Jack E Dixon
Feb 7, 2004·Science·Alexander F PalazzoGregg G Gundersen
May 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yuan GaoYi Zheng
Feb 8, 2005·Current Opinion in Microbiology·Ka-Wing Wong, Ralph R Isberg
Feb 25, 2005·Molecular Biology of the Cell·Shengli Hao, Avery August
Feb 26, 2005·Science·Luís Jaime MotaGuy R Cornelis
Mar 24, 2005·Infection and Immunity·Susan L Fink, Brad T Cookson
Apr 26, 2005·Annual Review of Microbiology·Gloria I Viboud, James B Bliska
Aug 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Markus C SchlumbergerWolf-Dietrich Hardt
Nov 22, 2005·Nature Methods·Jost EnningaGuy Tran Van Nhieu
May 23, 2006·Infection and Immunity·Ayelet ZaubermanBaruch Velan

❮ Previous
Next ❯

Citations

Apr 19, 2011·Annual Review of Phytopathology·Brad DayChristopher J Staiger
Aug 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Dara L BurdetteKim Orth
Jul 2, 2008·Immunologic Research·Matthew D Woolard, Jeffrey A Frelinger
Jan 1, 2011·Journal of Pathogens·Cristi L GalindoAshok K Chopra
Jan 25, 2011·European Journal of Cell Biology·Martin AepfelbacherKlaus Ruckdeschel
Jan 15, 2010·Current Opinion in Microbiology·Christopher A Broberg, Kim Orth
Dec 5, 2012·Molecular Microbiology·Rebecca DewoodyMelanie M Marketon
Jan 6, 2011·Molecular Microbiology·Rebecca DewoodyMelanie M Marketon
Jul 28, 2011·Cellular Microbiology·Rey Carabeo
Feb 13, 2010·Cellular Microbiology·Warangkhana SongsungthongJoan Mecsas
Jul 25, 2009·Cellular Immunology·Julie C WilliamsGlenn K Matsushima
Apr 9, 2015·Cellular Microbiology·Seblewongel AsratRalph R Isberg
May 26, 2010·Infection and Immunity·Tiffany M TsangEric S Krukonis
Oct 9, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anne DeuretzbacherKlaus Ruckdeschel
Jul 29, 2020·Immunological Reviews·Haleema S Malik, James B Bliska
Jun 28, 2013·The Journal of Biological Chemistry·Manuel WoltersMartin Aepfelbacher

❮ Previous
Next ❯

Datasets Mentioned

BETA
M17448

Methods Mentioned

BETA
GTPases
nucleotide
GTPase
transmission electron microscopy
confocal microscopy
pull-down
glucosylation
pull down
PCR
ELISA

Software Mentioned

Odyssey

Related Concepts

Related Feeds

Botulism (ASM)

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Botulism

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.