Yersinia enterocolitica infection and tcaA-dependent killing of Caenorhabditis elegans.

Applied and Environmental Microbiology
Britta SpanierThilo M Fuchs

Abstract

Caenorhabditis elegans is a validated model to study bacterial pathogenicity. We report that Yersinia enterocolitica strains W22703 (biovar 2, serovar O:9) and WA314 (biovar 1B, serovar O:8) kill C. elegans when feeding on the pathogens for at least 15 min before transfer to the feeding strain Escherichia coli OP50. The killing by Yersinia enterocolitica requires viable bacteria and, in contrast to that by Yersinia pestis and Yersinia pseudotuberculosis strains, is biofilm independent. The deletion of tcaA encoding an insecticidal toxin resulted in an OP50-like life span of C. elegans, indicating an essential role of TcaA in the nematocidal activity of Y. enterocolitica. TcaA alone is not sufficient for nematocidal activity because E. coli DH5alpha overexpressing TcaA did not result in a reduced C. elegans life span. Spatial-temporal analysis of C. elegans infected with green fluorescent protein-labeled Y. enterocolitica strains showed that Y. enterocolitica colonizes the nematode intestine, leading to an extreme expansion of the intestinal lumen. By low-dose infection with W22703 or DH5alpha followed by transfer to E. coli OP50, proliferation of Y. enterocolitica, but not E. coli, in the intestinal lumen of the nematode was ob...Continue Reading

References

Apr 1, 1990·Molecular Microbiology·R RosqvistH Wolf-Watz
Apr 1, 1985·Infection and Immunity·H Wolf-WatzS Falkow
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S C Straley, R R Brubaker
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J C Pepe, V L Miller
Aug 1, 1995·Trends in Microbiology·S C Straley, R D Perry
Apr 1, 1997·Clinical Microbiology Reviews·E J Bottone
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·M W TanF M Ausubel
Dec 22, 1999·Microbes and Infection·E J Bottone
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·C DarbyC Manoil
Sep 6, 2000·Molecular Microbiology·S Mahajan-MiklosF M Ausubel
Dec 15, 2000·Current Biology : CB·A LabrousseJ J Ewbank
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·D A GarsinF M Ausubel
Oct 10, 2001·Journal of Bacteriology·L A Gallagher, C Manoil
May 17, 2002·Nature·Creg DarbyStanley Falkow
Mar 26, 2003·Infection and Immunity·Costi D SifriStephen B Calderwood
Dec 4, 2003·Journal of Bacteriology·Damon W EllisonVirginia L Miller
Sep 3, 2004·Journal of Bacteriology·Vladimir L MotinEmilio Garcia
Sep 21, 2004·Nature Reviews. Microbiology·Nicholas R WaterfieldRichard H Ffrench-Constant
Nov 24, 2004·Microbiology and Immunology·Yanping HanRuifu Yang
Mar 2, 2005·Trends in Microbiology·Costi D SifriFrederick M Ausubel
Jan 5, 2006·Molecular Microbiology·Geraldine BresolinThilo M Fuchs
Jan 18, 2006·Current Opinion in Microbiology·Jürgen HeesemannKonrad Trülzsch
Feb 8, 2006·Applied and Environmental Microbiology·Line E ThomsenHanne Ingmer
Apr 6, 2006·Journal of Bacteriology·Geraldine BresolinThilo M Fuchs
May 16, 2006·Microbes and Infection·Marion S Dorer, Ralph R Isberg
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Terence I MoyFrederick M Ausubel
Feb 15, 2007·Environmental Microbiology·Hubert HilbiSimon Urwyler
Oct 31, 2007·Advances in Experimental Medicine and Biology·Nick WaterfieldRichard ffrench-Constant
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Theresa Stiernagle

❮ Previous
Next ❯

Citations

Nov 3, 2010·Applied and Environmental Microbiology·Shabarinath Srikumar, Thilo M Fuchs
Mar 23, 2013·Genome Génome / Conseil National De Recherches Canada·Shree P ThapaChun K Lim
Aug 29, 2013·ISRN Microbiology·Rui Zhang, Aixin Hou
Feb 20, 2014·Molecular Microbiology·Mandy Starke, Thilo M Fuchs
Apr 14, 2016·Proteomics. Clinical Applications·Nandini ChauhanJack C Leo
Dec 9, 2015·Infection and Immunity·Corin V WhiteMichael A Herman
Oct 5, 2019·Cellular and Molecular Life Sciences : CMLS·Arun KumarMojibur Khan
Nov 30, 2018·Frontiers in Cellular and Infection Microbiology·Katharina SpringerThilo M Fuchs

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

© 2021 Meta ULC. All rights reserved