Yersinia pseudotuberculosis disrupts intestinal barrier integrity through hematopoietic TLR-2 signaling.

The Journal of Clinical Investigation
Camille JungF Barreau

Abstract

Intestinal barrier function requires intricate cooperation between intestinal epithelial cells and immune cells. Enteropathogens are able to invade the intestinal lymphoid tissue known as Peyer's patches (PPs) and disrupt the integrity of the intestinal barrier. However, the underlying molecular mechanisms of this process are poorly understood. In mice infected with Yersinia pseudotuberculosis, we found that PP barrier dysfunction is dependent on the Yersinia virulence plasmid and the expression of TLR-2 by hematopoietic cells, but not by intestinal epithelial cells. Upon TLR-2 stimulation, Y. pseudotuberculosis-infected monocytes activated caspase-1 and produced IL-1β. In turn, IL-1β increased NF-κB and myosin light chain kinase activation in intestinal epithelial cells, thus disrupting the intestinal barrier by opening the tight junctions. Therefore, Y. pseudotuberculosis subverts intestinal barrier function by altering the interplay between immune and epithelial cells during infection.

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Citations

Jan 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·Naama KanarekYinon Ben-Neriah
Mar 29, 2014·Particle and Fibre Toxicology·Emilie BrunMarie Carrière
Apr 1, 2014·Cell·Yasmine Belkaid, Timothy W Hand
Jan 21, 2014·Current Opinion in Microbiology·F Barreau, J P Hugot
Apr 4, 2015·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Susan B FogelsonDaniel R Rissi
Mar 3, 2017·PLoS Pathogens·Ziad Al NabhaniFrederick Barreau
Mar 11, 2016·Inflammatory Bowel Diseases·Ziad AlnabhaniFrédérick Barreau
Dec 1, 2019·Journal of Crohn's & Colitis·Ziad Al NabhaniJean-Pierre Hugot
Mar 30, 2019·Journal of Intensive Care·Shunsuke Otani, Craig M Coopersmith
Jul 7, 2018·SAGE Open Medical Case Reports·Maria Belen Rojas GallegosDavid A Gremse
Feb 21, 2021·Journal of Crohn's & Colitis·Núria Solà TapiasFrédérick Barreau
Jul 25, 2021·International Journal of Molecular Sciences·Xavier Mas-OreaGilles Dietrich

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Methods Mentioned

BETA
IECs
electron microscopy
ELISA
GTPases
flow cytometry

Software Mentioned

Gatan
GraphPad Prism
GraphPad

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