Yersinia ruckeri lipopolysaccharide is necessary and sufficient for eliciting a protective immune response in rainbow trout (Oncorhynchus mykiss, Walbaum)

Fish & Shellfish Immunology
Timothy J Welch, S E LaPatra

Abstract

Enteric redmouth disease (ERM), caused by Yersinia ruckeri, has been controlled successfully using immersion-applied bacterin vaccines for several decades. While the host response to vaccination and the mechanism of protection of this vaccine have been elucidated, the bacterial components eliciting protection have remained unclear. Here we show that highly purified serotype O1 Y. ruckeri lipopolysaccharide (LPS) is sufficient to induce a protective response to experimental challenge in rainbow trout (Oncorhynchus mykiss). Dose response experiments demonstrated that Y. ruckeri LPS at doses of 1 ng/fish and above resulted in essentially complete protection and doses as low as 0.01 ng/fish (1.38 ng/kg) resulted in significant protection, thus demonstrating the extremely high potency of this immunogen. Analysis of the Y. ruckeri genome identified a cluster of putative O-antigen biosynthetic genes specific to serotype O1 strains. This cluster primarily consisted of genes encoding proteins predicted to function in the biosynthesis of legionamic acid, a nonulosonic acid known to be part of the O-polysaccharide repeat of O1 Y. ruckeri. Mutation of the nab2 gene, a nonulosonic acid biosynthesis gene (nab gene), resulted in production of...Continue Reading

References

May 16, 2007·Journal of Fish Diseases·E TobbackK Chiers
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Amanda L LewisAjit Varki
Dec 1, 2009·Nature Reviews. Microbiology·Clare E BryantNicholas J Gay
Dec 1, 2006·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Dimitar B IlievFrederick W Goetz
Mar 29, 2011·Applied and Environmental Microbiology·Timothy J WelchScott E LaPatra

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Citations

Oct 8, 2016·PloS One·Débora TorrealbaElena Garcia-Fruitós
Aug 23, 2020·Biology·Sascha R BrunnerBrian Dixon
Dec 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·David A Kennedy, Andrew F Read

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