Yes-associated protein promotes tumour necrosis factor α-treated cementoblast mineralization partly by inactivating NF-κB pathway.

Journal of Cellular and Molecular Medicine
Lu ZhangCui Huang

Abstract

Cementum regeneration, as one of the most difficult challenges of periodontal regeneration, is influenced by inflammatory factors. Inflammation may hamper or promote periodontal tissue repair under different circumstances, as it is found to do in dentin-pulp complex and bone tissue. Our team demonstrated that YAP promotes mineralization of OCCM, a cementoblast cell line. However, the effect of YAP on its mineralization under inflammatory microenvironment is unclear. In this study, cementogenesis in vitro was up-regulated after transient TNF-α treatment for 30 minutes. YAP expression also was increased by TNF-α treatment. YAP overexpression promoted OCCM mineralization after the cells were transiently treated with TNF-α because YAP overexpression inhibited NF-κB pathway activity, while YAP knockdown elevated it. The inhibited mineralization potential and activated NF-κB pathway activity by YAP knockdown also were partly rescued by the application of the NF-κB inhibitor Bay 11-7082. These results demonstrated that YAP plays a positive role in the mineralization of TNF-α transiently treated cementoblast, partly by inhibiting the NF-κB pathway activity.

References

Mar 29, 2011·Journal of Dental Research·M J B SilvaL Q Vieira
May 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jia ChangCun-Yu Wang
Apr 5, 2014·Journal of Endodontics·Paul R CooperAnthony J Smith
Jun 8, 2014·Archives of Oral Biology·Yong-Gun KimYoungkyun Lee
Mar 3, 2015·Biochemical and Biophysical Research Communications·Yuen GaoXiaodong Zhang
Jan 11, 2016·Journal of Biomedical Materials Research. Part a·Yingying ZhangYubo Fan
Jul 9, 2017·Journal of Cellular Physiology·Beining YangJiawei Wang
Jul 12, 2017·Journal of Dental Research·X WangZ Cao
Feb 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christopher D KegelmanJoel D Boerckel

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Methods Mentioned

BETA
transfections
Protein Assay
electrophoresis
transgenic
nuclear translocation

Software Mentioned

GraphPad Prism
ImageJ

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