YfmK is an Nε -lysine acetyltransferase that directly acetylates the histone-like protein HBsu in Bacillus subtilis

Proceedings of the National Academy of Sciences of the United States of America
Valerie J CarabettaDavid Dubnau

Abstract

Nε-lysine acetylation is an abundant and dynamic regulatory posttranslational modification that remains poorly characterized in bacteria. In bacteria, hundreds of proteins are known to be acetylated, but the biological significance of the majority of these events remains unclear. Previously, we characterized the Bacillus subtilis acetylome and found that the essential histone-like protein HBsu contains seven previously unknown acetylation sites in vivo. Here, we investigate whether acetylation is a regulatory component of the function of HBsu in nucleoid compaction. Using mutations that mimic the acetylated and unacetylated forms of the protein, we show that the inability to acetylate key HBsu lysine residues results in a more compacted nucleoid. We further investigated the mechanism of HBsu acetylation. We screened deletions of the ∼50 putative GNAT domain-encoding genes in B. subtilis for their effects on DNA compaction, and identified five candidates that may encode acetyltransferases acting on HBsu. Genetic bypass experiments demonstrated that two of these, YfmK and YdgE, can acetylate Hbsu, and their potential sites of action on HBsu were identified. Additionally, purified YfmK was able to directly acetylate HBsu in vitro,...Continue Reading

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J Rouvière-Yaniv, F Gros
Jul 1, 1987·Journal of Bacteriology·M AlbanoD Dubnau
Mar 1, 1997·Journal of Bacteriology·P Köhler, M A Marahiel
Jan 23, 1999·Molecular & General Genetics : MGG·P Köhler, M A Marahiel
Nov 8, 2002·The Journal of Biological Chemistry·Muyang LiWei Gu
Oct 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·J Spizizen
Nov 1, 2006·The Journal of Biological Chemistry·Maria SimonssonJohan Ericsson
Jan 16, 2007·Molecular & Cellular Proteomics : MCP·Boris MacekMatthias Mann
Feb 27, 2007·Cell·Tony Kouzarides
Oct 10, 2009·Nature Biotechnology·Karen T Smith, Jerry L Workman
Oct 31, 2009·Chemistry & Biology·Kinga Kamieniarz, Robert Schneider
Feb 9, 2010·Nature Reviews. Microbiology·Shane C Dillon, Charles J Dorman
Feb 16, 2011·Cell Research·Andrew J Bannister, Tony Kouzarides
Apr 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Alexander K W ElsholzUlf Gerth
Aug 7, 2012·Molecular & Cellular Proteomics : MCP·Amelia C PetersonJoshua J Coon
Nov 13, 2012·Journal of Bacteriology·Sampriti MukherjeeDaniel B Kearns
Jan 9, 2013·Journal of Proteome Research·Kai ZhangZhongyi Cheng
Jul 9, 2013·Molecular Cell·Brian T WeinertChunaram Choudhary
Aug 2, 2013·Journal of Proteome Research·Hiroki OkanishiSeiki Kuramitsu
Mar 19, 2014·New Biotechnology·Vicente BernalManuel Cánovas
Apr 15, 2014·Molecular Cell·Gregory R Wagner, Matthew D Hirschey
Jun 12, 2014·The Journal of Biological Chemistry·Josue BaezaJohn M Denu
Dec 3, 2014·The International Journal of Biochemistry & Cell Biology·Longxiang XieJianping Xie
Jul 17, 2015·Microbiology and Molecular Biology Reviews : MMBR·Kristy L Hentchel, Jorge C Escalante-Semerena
Sep 4, 2016·Journal of the American Society for Mass Spectrometry·Jesse G MeyerBirgit Schilling
Apr 26, 2017·Journal of Bacteriology·Valerie J Carabetta, Ileana M Cristea

❮ Previous
Next ❯

Citations

Jul 8, 2020·Molecular Systems Biology·Willy V BienvenutIris Finkemeier
Sep 24, 2020·Journal of Proteome Research·Valerie J Carabetta
Jul 18, 2020·Journal of Proteome Research·Jakub NovakPeter Sebo
Jul 13, 2021·Journal of Bacteriology·Aiswarya Dash, Rahul Modak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.