YFR016c/Aip5 is part of an actin nucleation complex in yeast

Biology Open
Oliver GlombNils Johnsson

Abstract

The polarisome comprises a network of proteins that organizes polar growth in yeast and filamentous fungi. The yeast formin Bni1 and the actin nucleation-promoting factor Bud6 are subunits of the polarisome that together catalyze the formation of actin cables below the tip of yeast cells. We identified YFR016c (Aip5) as an interaction partner of Bud6 and the polarisome scaffold Spa2. Yeast cells lacking Aip5 display a reduced number of actin cables. Aip5 binds with its N-terminal region to Spa2 and with its C-terminal region to Bud6. Both interactions collaborate to localize Aip5 at bud tip and neck, and are required to stimulate the formation of actin cables. Our experiments characterize Aip5 as a novel subunit of a complex that regulates the number of actin filaments at sites of polar growth.

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Citations

Aug 28, 2020·The Journal of Biological Chemistry·Ying XieYansong Miao
Jan 10, 2021·Journal of Cell Science·Ying Xie, Yansong Miao
Mar 4, 2021·The Journal of Cell Biology·Alexander DünklerNils Johnsson
Feb 27, 2021·Frontiers in Cell and Developmental Biology·Thomas J Rands, Bruce L Goode

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Methods Mentioned

BETA
GTPase
fluorescence microscopy
PCR

Software Mentioned

Zen2
FIJI
Plot Profile ’

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