YghG (GspSβ) is a novel pilot protein required for localization of the GspSβ type II secretion system secretin of enterotoxigenic Escherichia coli.

Infection and Immunity
Timothy G StrozenS Peter Howard

Abstract

The enterotoxigenic Escherichia coli (ETEC) pathotype, characterized by the prototypical strain H10407, is a leading cause of morbidity and mortality in the developing world. A major virulence factor of ETEC is the type II secretion system (T2SS) responsible for secretion of the diarrheagenic heat-labile enterotoxin (LT). In this study, we have characterized the two type II secretion systems, designated alpha (T2SS(α)) and beta (T2SS(β)), encoded in the H10407 genome and describe the prevalence of both systems in other E. coli pathotypes. Under laboratory conditions, the T2SS(β) is assembled and functional in the secretion of LT into culture supernatant, whereas the T2SS(α) is not. Insertional inactivation of the three genes located upstream of gspC(β) (yghJ, pppA, and yghG) in the atypical T2SS(β) operon revealed that YghJ is not required for assembly of the GspD(β) secretin or secretion of LT, that PppA is likely the prepilin peptidase required for the function of T2SS(β), and that YghG is required for assembly of the GspD(β) secretin and thus function of the T2SS(β). Mutational and physiological analysis further demonstrated that YghG (redesignated GspS(β)) is a novel outer membrane pilotin protein that is integral for assem...Continue Reading

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Citations

Apr 2, 2013·Research in Microbiology·S Peter Howard
Jul 31, 2013·Acta Crystallographica. Section D, Biological Crystallography·Saima RehmanRichard W Pickersgill
Jul 4, 2013·Journal of Structural Biology·Konstantin V KorotkovWim G J Hol
Sep 3, 2016·PLoS Genetics·Jakob Frimodt-MøllerAnders Løbner-Olesen
Apr 6, 2017·Nucleic Acids Research·Sinyeon KimSung Ho Yoon
Mar 8, 2017·Infection and Immunity·Nicholas P Cianciotto, Richard C White
Apr 8, 2014·Expert Review of Vaccines·James FleckensteinFirdausi Qadri
Feb 6, 2020·Protein Science : a Publication of the Protein Society·Yuri Rafael de Oliveira SilvaAndréa Dessen
May 10, 2017·Molecular Microbiology·Jenny-Lee ThomassinOlivera Francetic
Dec 8, 2020·Molecular Microbiology·Souvik NaskarHarry H Low
Feb 16, 2019·EcoSal Plus·Konstantin V Korotkov, Maria Sandkvist

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