Yin Yang 1, Oct1, and NFAT-4 form repeating, cyclosporin-sensitive regulatory modules within the murine CD21 intronic control region

The Journal of Immunology : Official Journal of the American Association of Immunologists
Mark D ZabelJohn H Weis

Abstract

The murine complement receptor type 2 gene (Cr2/CD21) is expressed by murine B and follicular dendritic cells, but not murine T cells. We have previously shown that appropriate transcriptional control of the CD21 gene requires the CD21 promoter as well as intronic sequences. We have also demonstrated that altering chromatin structure by inhibiting histone deacetylases induces CD21 expression in murine T cells by increasing the accessibility of promoter and intronic regulatory elements. In this report, we identify seven distinct regulatory areas within the first intron of the murine CD21 gene that are conserved between mouse and human CD21 intronic sequences. EMSA competition and supershift analyses reveal the formation of multiple DNA-protein complexes at these sites that include Yin Yang 1, Oct1, and NFAT-4. NFAT-containing complexes were altered in B cells treated with the NFAT inhibitor cyclosporin A and correlated with a repression of CD21 gene transcription implicating NFAT transcriptional control. Functional data revealed that no single region conferred cell-specific reporter gene expression, but rather the entire CD21 regulatory element was required to confer cell-specific gene expression. Taken together, these data demo...Continue Reading

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Citations

Oct 6, 2007·Immunologic Research·Brian K Martin
Aug 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carol B Martin, Brian K Martin
Nov 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jackie G LeeRajabrata Sarkar
Nov 6, 2010·The Journal of Biological Chemistry·Arvind ShakyaDean Tantin
Aug 27, 2009·Human Molecular Genetics·Patricia KieslerDonata Vercelli
Jun 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kazuyuki YokoyamaMasanori Hatakeyama
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Irina DebnathJohn H Weis

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