Yolkin Isolated from Hen Egg Yolk as a Natural Immunoregulator, Activating Innate Immune Response in BMDM Macrophages

Oxidative Medicine and Cellular Longevity
Wioletta KazanaAgnieszka Zabłocka

Abstract

One of the goals of biomedical sciences is to search and identify natural compounds that are safe, have no side effects, and possess immunostimulatory activity. It has been proven that medicines of natural origin can be effective agents, supporting the therapy of many diseases, not only in the weakened immune system of the body but also in the prevention of many diseases in healthy people. It has been shown that yolkin, a polypeptide complex isolated from hen egg yolk as a fraction accompanying immunoglobulin Y (IgY), possesses potential biological activity. However, the mechanism of its action has not been explained. The objective of this investigation was to examine the molecular mechanisms of innate immune response, activated in response to yolkin, in murine bone marrow-derived macrophages (BMDM). It was shown that yolkin induced phosphorylation of extracellular signal-kinases (ERK1/2) and c-Jun N-terminal kinase (JNK) and upregulated expression and production of type I interferons, TNF-α (tumor necrosis factor α), and nitric oxide (NO), in BMDM cells. Using pharmacological inhibitors of ERK 1/2 and JNK kinases, we revealed that the JNK signaling cascade is required for yolkin-induced inducible NOS expression and upregulatio...Continue Reading

References

Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C J LowensteinW J Murphy
Aug 7, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·I GuevaraA Szczudlik
Dec 7, 2000·Immunology·T Akaike, H Maeda
Jun 29, 2002·Current Opinion in Immunology·Martín E RottenbergHans Wigzell
Jul 2, 2004·Nature Reviews. Immunology·Shizuo Akira, Kiyoshi Takeda
Oct 27, 2005·Journal of Agricultural and Food Chemistry·Jennifer Kovacs-NolanYoshinori Mine
Aug 12, 2008·Ageing Research Reviews·Hae Young ChungChristiaan Leeuwenburgh
Nov 20, 2008·Current Protocols in Immunology·David M Mosser, Xia Zhang
Dec 25, 2008·Annual Review of Immunology·Fernando O MartinezSiamon Gordon
Sep 18, 2009·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Agnieszka ZabłockaMaria Janusz
Dec 1, 2010·Immunity & Ageing : I & a·Yazdani B Shaik-DasthagirisahebFrank C Gibson
Apr 19, 2011·International Immunopharmacology·Jong Seok Lee, Eock Kee Hong
Nov 17, 2012·Poultry Science·A PolanowskiT Trziszka
Oct 26, 2013·Nature Reviews. Immunology·Albert C ShawRuth R Montgomery
Jan 15, 2014·International Journal of Biological Macromolecules·Ting ZhaoXiangyang Wu
Jan 1, 2014·Postȩpy higieny i medycyny doświadczalnej·Aleksandra ZambrowiczMarek Szołtysik
Jul 26, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Elaine Uchima UeharaCyro Alves de Brito
Oct 2, 2015·Current Opinion in Pharmacology·Joachim L Schultze
Jan 5, 2016·Current Neuropharmacology·Vivekkumar P DadhaniaDurga Nand Tripathi
Feb 6, 2016·British Journal of Pharmacology·A G Stewart, P M Beart
Feb 24, 2016·International Immunopharmacology·Luxi Chen, Jianhua Yu
Jun 14, 2016·Mediators of Inflammation·Monica MolteniCarlo Rossetti
Dec 13, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Sahil KumarT R Bhardwaj
Mar 28, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·J Martin BrownSamuel Strober
Apr 23, 2017·Cellular and Molecular Life Sciences : CMLS·Nicola Raftery, Nigel J Stevenson
Aug 6, 2017·Results and Problems in Cell Differentiation·Hongyan Li, Shicui Zhang
Jan 11, 2018·Journal of Cellular Physiology·Abbas Shapouri-MoghaddamAmirhossein Sahebkar
Mar 5, 2018·Cellular and Molecular Life Sciences : CMLS·Yannic Nonnenmacher, Karsten Hiller
Jun 21, 2018·International Journal of Molecular Sciences·Chiraz AtriDhafer Laouini
Sep 2, 2018·International Journal of Biological Macromolecules·Dan-Dan WangYan Chen

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Methods Mentioned

BETA
ELISA
PCR
enzyme-linked immunosorbent assay
Assay
size-exclusion chromatography

Software Mentioned

Statistica
StatSoft

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