PMID: 9440524Jan 24, 1998Paper

YopD of Yersinia pestis plays a role in negative regulation of the low-calcium response in addition to its role in translocation of Yops

Journal of Bacteriology
A W Williams, S C Straley

Abstract

Yersinia pestis produces a set of virulence proteins (Yops and LcrV) that are expressed at high levels and secreted by a type III secretion system (Ysc) upon bacterium-host cell contact, and four of the Yops are vectorially translocated into eukaryotic cells. YopD, YopB, and YopK are required for the translocation process. In vitro, induction and secretion occur at 37 degrees C in the absence of calcium. LcrH (also called SycD), a protein required for the stability and secretion of YopD, had initially been identified as a negative regulator of Yop expression. In this study, we constructed a yopD mutation in both wild-type and secretion-defective (ysc) Y. pestis to determine if the lcrH phenotype could be attributed to the decreased stability of YopD. These mutants were constitutively induced for expression of Yops and LcrV, despite the presence of the secreted negative regulator LcrQ, demonstrating that YopD is involved in negative regulation, regardless of a functioning Ysc system. Normally, secretion of Yops and LcrV is blocked in the presence of calcium. The single yopD mutant was not completely effective in blocking secretion: LcrV was secreted equally well in the presence and absence of calcium, while there was partial sec...Continue Reading

References

Nov 1, 1991·Journal of Bacteriology·G V PlanoS C Straley
Oct 1, 1989·Journal of Bacteriology·S B PriceS C Straley
Oct 1, 1988·Infection and Immunity·O A Sodeinde, J D Goguen
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Mar 1, 1984·Infection and Immunity·T Une, R R Brubaker
Feb 1, 1981·Infection and Immunity·D M Ferber, R R Brubaker
Jan 1, 1995·Antimicrobial Agents and Chemotherapy·C LévesqueP H Roy
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·P WattiauG R Cornelis
Jan 1, 1993·Infection and Immunity·S HåkanssonH Wolf-Watz
Jan 1, 1993·Trends in Biochemical Sciences·G P Salmond, P J Reeves
Apr 1, 1993·Protein Expression and Purification·J T MooreG F Maley
Jun 1, 1993·Journal of Bacteriology·G V Plano, S C Straley
Jun 1, 1996·Infection and Immunity·E L HartlandR M Robins-Browne
Jan 1, 1997·Clinical Microbiology Reviews·R D Perry, J D Fetherston
Mar 1, 1997·Infection and Immunity·J Nemeth, S C Straley
Mar 1, 1997·Molecular Microbiology·G R Cornelis, H Wolf-Watz
Sep 1, 1997·Infection and Immunity·K A FieldsS C Straley

❮ Previous
Next ❯

Citations

Oct 18, 2000·Infection and Immunity·J B DayG V Plano
May 25, 2002·Journal of Bacteriology·Kumaran S Ramamurthi, Olaf Schneewind
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Kumaran S Ramamurthi, Olaf Schneewind
Mar 20, 2003·Journal of Bacteriology·Petra J EdqvistScott A Lloyd
Aug 11, 2005·Molecular Microbiology·Franco FerracciGregory V Plano
Apr 24, 2008·Molecular Microbiology·C A MuellerG R Cornelis
Nov 4, 2008·Molecular Microbiology·Bill BlaylockOlaf Schneewind
Sep 21, 2010·Journal of Bacteriology·Cindy J Gode-PotratzLinda L McCarter
Dec 9, 2015·Journal of Bacteriology·Leah SchwiesowVictoria Auerbuch
Aug 8, 2001·Journal of Bacteriology·J S Matson, M L Nilles
Oct 9, 2002·Molecular Microbiology·Anne-Laure Page, Claude Parsot
Apr 20, 2001·Molecular Microbiology·G V PlanoF Ferracci
Feb 24, 2001·Cellular Microbiology·A BlockerG Cornelis
May 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Arne RietschJohn J Mekalanos
Oct 29, 2004·The Journal of Biological Chemistry·Jason A RosenzweigKurt Schesser
Sep 2, 2005·Molecular Microbiology·Julie TorruellasGregory V Plano
Feb 27, 2009·The Journal of Biological Chemistry·Annika SchmidGottfried Wilharm
Jun 8, 2010·The Journal of Biological Chemistry·Tiago R D CostaMatthew S Francis
Jan 30, 2013·Infection and Immunity·Andrew S HouppertMelanie M Marketon
Feb 22, 2017·MSphere·Jessica M MorganVictoria Auerbuch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.