YopM of Yersinia enterocolitica specifically interacts with alpha1-antitrypsin without affecting the anti-protease activity

Microbiology
Gerhard HeusippM Alexander Schmidt

Abstract

It was previously shown that alpha1-antitrypsin (AAT) interacts with the type III secreted (T3S) EspB and EspD proteins of enteropathogenic Escherichia coli (EPEC), resulting in reduced functionality of the proteins. To determine if AAT is also able to interact with T3S proteins of other pathogens, the binding of AAT to Yop proteins of Yersinia enterocolitica was analysed. AAT did not interact with YopB or YopD, which have functions in type III translocation similar to EspB and EspD in EPEC, but specifically interacts with YopM, a member of the leucine-rich repeat (LRR) family of proteins, in overlay and pull-down assays. To determine regions of YopM involved in AAT binding, various N- and C-terminally truncated versions of YopM were recombinantly expressed, and their ability to interact with AAT analysed. All versions tested were able to bind AAT, indicating that at least eight LRR of YopM are sufficient for AAT interaction. The main physiological role of AAT is to inhibit neutrophil elastase; however, elastase was efficiently inhibited by AAT in the presence and absence of YopM, indicating that YopM does not interfere with the anti-protease inhibition activity of AAT, and that the domain of AAT interacting with YopM is not id...Continue Reading

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Citations

Dec 22, 2007·American Journal of Respiratory Cell and Molecular Biology·Taras ManolovKristian Riesbeck
Mar 19, 2014·International Journal of Medical Microbiology : IJMM·Christian RüterM Alexander Schmidt
Apr 14, 2015·International Journal of Medical Microbiology : IJMM·Sabrina HöflingChristian Rüter
Dec 5, 2009·Molecular Plant-microbe Interactions : MPMI·Mandy WenzelSusanne Zehner
Mar 17, 2016·World Journal of Biological Chemistry·Khavong Pha, Lorena Navarro
Aug 24, 2018·Cellular Microbiology·Stefanie NorkowskiChristian Rüter
Jul 10, 2013·Cellular and Molecular Life Sciences : CMLS·Julia ScharnertChristian Rüter

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