"You produce while I clean up", a strategy revealed by exoproteomics during Synechococcus-Roseobacter interactions

Proteomics
Joseph A Christie-OlezaJean Armengaud

Abstract

Most of the energy that is introduced into the oceans by photosynthetic primary producers is in the form of organic matter that then sustains the rest of the food web, from micro to macro-organisms. However, it is the interactions between phototrophs and heterotrophs that are vital to maintaining the nutrient balance of marine microbiomes that ultimately feed these higher trophic levels. The primary produced organic matter is mostly remineralized by heterotrophic microorganisms but, because most of the oceanic dissolved organic matter is in the form of biopolymers, and microbial membrane transport systems operate with molecules <0.6 kDa, it must be hydrolyzed outside the cell before a microorganism can acquire it. As a simili of the marine microbiome, we analyzed, using state-of-the-art proteomics, the exoproteomes obtained from synthetic communities combining specific Roseobacter (Ruegeria pomeroyi DSS-3, Roseobacter denitrificans OCh114, and Dinoroseobacter shibae DFL-12) and Synechococcus strains (WH7803 and WH8102). This approach identified the repertoire of hydrolytic enzymes secreted by Roseobacter, opening up the black box of heterotrophic transformation/remineralization of biopolymers generated by marine phytoplankton. ...Continue Reading

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Citations

Feb 18, 2016·The ISME Journal·Winifred M JohnsonElizabeth B Kujawinski
Sep 30, 2015·Proteomics·Joseph A Christie-Oleza, Jean Armengaud
Jul 5, 2016·Frontiers in Microbiology·Joaquín Giner-LamiaPaulo Oliveira
Dec 15, 2015·Environmental Microbiology·Paulo OliveiraPaula Tamagnini
Jun 27, 2017·Nature Microbiology·Joseph A Christie-OlezaDavid J Scanlan
Dec 2, 2017·Environmental Microbiology·Amandeep KaurJoseph A Christie-Oleza
Nov 19, 2017·Applied and Environmental Microbiology·Qiang ZhengNianzhi Jiao
Nov 11, 2018·Applied and Environmental Microbiology·Christian ZerfaßOrkun S Soyer
Feb 23, 2020·Environmental Microbiology·Vinko ZadjelovicJoseph A Christie-Oleza
Nov 12, 2019·Frontiers in Microbiology·Kate L HarveySteven P Djordjevic
Nov 12, 2020·Nature Communications·Emily J ZakemMichael J Follows
Mar 27, 2021·Nature Communications·Maria Del Mar Aguilo-FerretjansJoseph A Christie-Oleza
May 28, 2021·Frontiers in Microbiology·Abbrar LabbanXosé Anxelu G Morán
May 29, 2021·Environmental Science. Nano·Craig J DedmanGemma-Louise Davies
Jan 3, 2020·Environmental Science & Technology·Robyn J WrightJoseph A Christie-Oleza
Sep 10, 2021·Microbiology Resource Announcements·Ruiyu ZhouRui Wang

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Methods Mentioned

BETA
flow cytometry
transmission electron microscopy
GTAs

Software Mentioned

Mascot
Roseo BLASTp
BioEdit BLAST
Scaffold
BLASTp
Proteome
Matrix Science

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