YrhB is a highly stable small protein with unique chaperone-like activity in Escherichia coli BL21(DE3)

FEBS Letters
Keum-Young AhnJeewon Lee

Abstract

Escherichia coli YrhB (10.6 kDa) from strain BL21(DE3) that is commonly used for protein overexpression is a stable chaperone-like protein and indispensable for supporting the growth of BL21(DE3) at 48 °C but not defined as conventional heat shock protein (HSP). YrhB effectively prevented heat-induced aggregation of ribonucleotide synthetase (PurK). Without ATP, YrhB alone promoted in vitro refolding of uridine phosphorylase (UDP) and protected thermal denaturation of the refolded UDP. As a cis-acting fusion partner, YrhB also significantly reduced inclusion body formation of nine aggregation-prone heterologous proteins in BL21(DE3). Unlike conventional small HSPs, YrhB remained monomer under heat shock condition.

References

May 2, 1977·European Journal of Biochemistry·J C LeerM Schwartz
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G H Lorimer
May 1, 1997·Biochemistry and Molecular Biology International·B I KurganovV G Debabov
Feb 12, 1998·The Journal of Biological Chemistry·H J OhJ R Subjeck
Apr 16, 1998·Trends in Biochemical Sciences·W J Netzer, F U Hartl
Feb 20, 1999·Cell·U JakobJ C Bardwell
Dec 3, 1999·The EMBO Journal·M HaslbeckJ Buchner
Nov 10, 2001·Nature Structural Biology·R L van MontfortE Vierling
Mar 9, 2002·Science·F Ulrich Hartl, Manajit Hayer-Hartl
May 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ronald S UllersPierre Genevaux
Nov 6, 2004·Nature Biotechnology·François Baneyx, Mirna Mujacic
Jan 25, 2008·The EMBO Journal·Krzysztof LiberekSzymon Zietkiewicz
Mar 28, 2008·Journal of Proteome Research·Kyung-Yeon HanJeewon Lee
Nov 3, 2009·Nature Biotechnology·Byung-Kwan ChoBernhard Ø Palsson
May 13, 2010·Molecular Systems Biology·Szymon JozefczukLothar Willmitzer

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Citations

Jul 16, 2013·The Analyst·Dae-Sung LeeJeewon Lee

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