YtkD and MutT protect vegetative cells but not spores of Bacillus subtilis from oxidative stress

Journal of Bacteriology
Francisco X Castellanos-JuárezMario Pedraza-Reyes

Abstract

ytkD and mutT of Bacillus subtilis encode potential 8-oxo-dGTPases that can prevent the mutagenic effects of 8-oxo-dGTP. Loss of YtkD but not of MutT increased the spontaneous mutation frequency of growing cells. However, cells lacking both YtkD and MutT had a higher spontaneous mutation frequency than cells lacking YtkD. Loss of either YtkD or MutT sensitized growing cells to hydrogen peroxide (H2O2) and t-butylhydroperoxide (t-BHP), and the lack of both proteins sensitized growing cells to these agents even more. In contrast, B. subtilis spores lacking YtkD and MutT were not sensitized to H2O2, t-BHP, or heat. These results suggest (i) that YtkD and MutT play an antimutator role and protect growing cells of B. subtilis against oxidizing agents, and (ii) that neither YtkD nor MutT protects spores against potential DNA damage induced by oxidative stress or heat.

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Citations

Oct 26, 2010·Journal of Bacteriology·Bernardo N DeboraMario Pedraza-Reyes
Sep 11, 2012·Journal of Bacteriology·Fernando H Ramírez-GuadianaMario Pedraza-Reyes
Aug 31, 2012·Microbiology and Molecular Biology Reviews : MMBR·Justin S LenhartLyle A Simmons
Oct 6, 2007·Critical Reviews in Biochemistry and Molecular Biology·Eduardo A RobletoMario Pedraza-Reyes
Jun 29, 2014·Applied and Environmental Microbiology·Fernando Santos-EscobarMario Pedraza-Reyes
Jan 29, 2020·The Journal of Microbiology·Ozan ErtekinGülay Özcengiz
Feb 12, 2020·Journal of Bacteriology·Hilda C Leyva-SánchezMario Pedraza-Reyes
May 19, 2020·MicrobiologyOpen·Joanna DrabinskaElzbieta Kraszewska
Dec 1, 2020·Microbiology·Elzbieta Kraszewska, Joanna Drabinska
Jun 25, 2015·Microbiology Spectrum·Peter Setlow

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