Yucasin DF, a potent and persistent inhibitor of auxin biosynthesis in plants

Scientific Reports
Shinichi TsugafuneKen-Ichiro Hayashi

Abstract

The plant hormone auxin plays a crucial role in plant growth and development. Indole-3-acetic acid (IAA), a natural auxin, is mainly biosynthesized by two sequential enzyme reactions catalyzed by TAA1 and YUCCA (YUC). TAA1 is involved in the conversion of tryptophan to IPA, and YUC catalyzes the conversion of IPA to IAA. We previously demonstrated that yucasin inhibits AtYUC1 enzyme activity and suppress high-auxin phenotype of YUC overexpression plants, although yucasin displayed weak effects on the auxin-related phenotype of wild-type plants. To develop more potent YUC inhibitors, various derivatives of yucasin were synthesized, and their structure-activity relationships were investigated. Yucasin difluorinated analog (YDF) (5-[2,6-difluorophenyl]-2,4-dihydro-[1,2,4]-triazole-3-thione) was identified to be a more potent YUC inhibitor than the original yucasin. YDF caused an auxin-deficient phenotype in Arabidopsis wild-type plants that was restored with auxin application. YDF was found to be highly stable regarding metabolic conversion in vivo, accounting for the potent activity of the inhibition of IAA biosynthesis in planta. Photoaffinity labeling experiments demonstrated that yucasin-type inhibitors bind to the active site...Continue Reading

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Citations

Apr 19, 2018·Plant & Cell Physiology·Kosuke Fukui, Ken-Ichiro Hayashi
Sep 1, 2018·Plant & Cell Physiology·Kiyoshi MashiguchiHiroyuki Kasahara
Nov 23, 2018·International Journal of Molecular Sciences·Hua Qin, Rongfeng Huang
Aug 5, 2020·Nature Plants·Rotem MatosevichIdan Efroni
Jul 9, 2020·International Journal of Molecular Sciences·Miguel A Uc-ChucVíctor M Loyola-Vargas
Jul 23, 2020·Plant & Cell Physiology·Shutaro KanekoHiroyuki Kasahara
Jan 20, 2021·Plant, Cell & Environment·Aurora Alaguero-CordovillaJosé Manuel Pérez-Pérez
Feb 16, 2021·Biotechnology Advances·Caroline E PaulWillem J H van Berkel
Jan 13, 2021·Cold Spring Harbor Perspectives in Biology·Hidemasa SuzukiRyuichi Nishihama
Jan 13, 2021·Cold Spring Harbor Perspectives in Biology·Ken-Ichiro Hayashi
Sep 26, 2021·The Plant Journal : for Cell and Molecular Biology·Logan DeMottMaeli Melotto

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Methods Mentioned

BETA
transgenic
fluorescence microscopy
fluorescence imaging

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