Abstract
ZAP-70 expression is a strong prognostic indicator in chronic lymphocytic leukemia. However, ZAP-70 quantification by flow cytometry lacks sufficient standardization. Based upon the correlation between ZAP-70 expression and its gene methylation status, we have developed a quantitative pyrosequencing assay for the determination of ZAP-70 methylation adapted for routine use. Methylation in four CpG pairs (C-223, C-243, C-254, and C-267) in the first intron of ZAP-70 is associated with repression of ZAP-70. Moreover, it correlates with CD38 expression (n=111, p<.0001), IgHv mutation status (n=106, p<.0001), time to treatment (p<.0001), and overall survival (p=.0014). Pyrosequencing of ZAP-70 provides a good alternative to flow cytometry.
References
Nov 13, 1992·Cell·A C ChanA Weiss
Jul 1, 1981·Cancer·J L BinetF Gremy
Aug 11, 1994·Nucleic Acids Research·S J ClarkM Frommer
Mar 11, 1994·Cell·E ArpaiaC M Roifman
Jun 10, 1994·Science·A C ChanA Weiss
Jan 11, 2000·Nucleic Acids Research·C GrunauG Roizes
Jan 3, 2001·The New England Journal of Medicine·H DöhnerP Lichter
Dec 26, 2001·The Journal of Experimental Medicine·U KleinR Dalla-Favera
Dec 26, 2001·The Journal of Experimental Medicine·A RosenwaldL M Staudt
Jan 25, 2002·Blood·Terry J HamblinDavid G Oscier
Jun 4, 2002·Nature Reviews. Genetics·Peter A Jones, Stephen B Baylin
Feb 22, 2003·Blood·Adrian WiestnerLouis M Staudt
May 2, 2003·The New England Journal of Medicine·Marta CrespoEmili Montserrat
Oct 3, 2003·Leukemia·J DürigU Dührsen
Jan 17, 2004·Lancet·Jenny A OrchardDavid G Oscier
May 11, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Joan H AdmirandL Jeffrey Medeiros
Aug 25, 2004·Hematology/oncology Clinics of North America·Kanti R RaiBhoomi Mehrotra
Aug 27, 2004·The New England Journal of Medicine·Laura Z RassentiThomas J Kipps
Feb 25, 2005·The New England Journal of Medicine·Nicholas ChiorazziManlio Ferrarini
Apr 1, 2005·Leukemia·J C NolzD F Jelinek
Feb 10, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marta CrespoFrancesc Bosch
Feb 17, 2006·Leukemia·C ScielzoP Ghia
Apr 8, 2006·Blood·Maria Ilaria Del PrincipeSergio Amadori
Dec 13, 2006·Genes & Development·Angela H TingStephen B Baylin
Dec 15, 2006·Leukemia·P GhiaUNKNOWN European Research Initiative on CLL
Sep 26, 2007·Human Molecular Genetics·Manel Esteller
Aug 19, 2007·Blood·Silvia DeaglioFabio Malavasi
Jan 29, 2008·Haematologica·Nathalie GachardUNKNOWN GEIL
Jun 26, 2008·Blood·Laura Z RassentiThomas J Kipps
Sep 2, 2008·Haematologica·Cristina Reinoso-MartínJavier García-Conde
Apr 21, 2009·Haematologica·Basile StamatopoulosLaurence Lagneaux
Citations
Jun 22, 2011·Clinical and Experimental Medicine·Yin-Hua WangJian-Yong Li
Apr 30, 2010·Apoptosis : an International Journal on Programmed Cell Death·Roman ThalerFranz Varga
Aug 28, 2012·Leukemia·N CahillR Rosenquist
May 9, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Rainer ClausJohn C Byrd
Dec 1, 2011·Epigenomics·Cristina FloreanMarc Diederich
Feb 28, 2015·Leukemia & Lymphoma·David M LucasMichael R Grever
Dec 14, 2011·Journal of Cellular and Molecular Medicine·Shilu AminJelena Mann
Jan 1, 2014·Molecular Cancer·Lu Qian WangChor Sang Chim
Apr 27, 2012·Epigenetics : Official Journal of the DNA Methylation Society·Lirong PeiHuidong Shi
Jan 17, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Nicola Cahill, Richard Rosenquist
May 29, 2014·Blood·Rainer ClausJohn C Byrd
Jan 30, 2015·International Journal of Hematology·David RodríguezCarlos López-Otín
Apr 30, 2016·Epigenetics : Official Journal of the DNA Methylation Society·Sujata BhoiLarry Mansouri
Aug 25, 2010·Advances in Anatomic Pathology·Michele Roullet, Adam Bagg
Mar 20, 2020·Genome Medicine·Justyna A WierzbinskaDaniel B Lipka
Aug 18, 2020·Future Science OA·Hanaa Rm AttiaMohamed Emam Sobeih
Mar 15, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Oscar MurciaAjay Goel