ZBP1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons

Scientific Reports
Anna S UrbanskaJacek Jaworski

Abstract

Local protein synthesis occurs in axons and dendrites of neurons, enabling fast and spatially restricted responses to a dynamically changing extracellular environment. Prior to local translation, mRNA that is to be translated is packed into ribonucleoprotein particles (RNPs) where RNA binding proteins ensure mRNA silencing and provide a link to molecular motors. ZBP1 is a component of RNP transport particles and is known for its role in the local translation of β-actin mRNA. Its binding to mRNA is regulated by tyrosine 396 phosphorylation, and this particular modification was shown to be vital for axonal growth and dendritic branching. Recently, additional phosphorylation of ZBP1 at serine 181 (Ser181) was described in non-neuronal cells. In the present study, we found that ZBP1 is also phosphorylated at Ser181 in neurons in a mammalian/mechanistic target of rapamycin complex 2-, Src kinase-, and mRNA binding-dependent manner. Furthermore, Ser181 ZBP1 phosphorylation was essential for the proper dendritic branching of hippocampal neurons that were cultured in vitro and for the proper ZBP1 dendritic distribution and motility.

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Citations

Apr 12, 2018·Biological Chemistry·Fabienne De Graeve, Florence Besse
Jun 2, 2018·FEBS Letters·Christopher Gallagher, Andres Ramos
Mar 21, 2020·Journal of Neurochemistry·Aleksandra TempesJacek Jaworski
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Jun 27, 2020·Trends in Endocrinology and Metabolism : TEM·Ning Dai
Jul 13, 2021·Investigational New Drugs·Qiu-Ying DuDong-Sheng Pei

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Methods Mentioned

BETA
transfection
RNA-IP
PCR
pulldown
immunoprecipitation
immunprecipitation
co-immunoprecipitation

Software Mentioned

OpenOffice Calc
GraphPad
MetaMorph
Prism

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