ZBTB16 induces osteogenic differentiation marker genes in dental follicle cells independent from RUNX2

Journal of Periodontology
Oliver FelthausChristian Morsczeck

Abstract

Dental follicle cells (DFCs) are neural crest cell-derived cells and the genuine precursor cells of cementoblast and alveolar osteoblasts. After osteogenic differentiation, expression levels of the transcription factor zinc factor and BTB domain containing 16 (ZBTB16) were significantly increased. ZBTB16 is associated with the process of osteogenic differentiation in bone marrow-derived mesenchymal stem cells and crucial for the expression of the osteogenic transcription factor runt-related transcription factor 2 (RUNX2). It is proposed that ZBTB16 plays also a crucial role for the differentiation of DFCs into osteoblasts. In this study, the differentiation of DFCs by alkaline phosphatase (ALP) activity measurement, alizarin red staining, and electron-dispersive x-ray spectrometry (EDX) analysis is investigated. The expression of ZBTB16 during osteogenic differentiation and the expression of osteogenic differentiation markers were assessed by real-time reverse transcription polymerase chain reaction. Glucocorticoid stimulation was inhibited using RU486 (11β-[p-(Dimethylamino)phenyl]-17β-hydroxy-17-(1-propynyl)estra-4,9-dien-3-one), and ZBTB16 was overexpressed via transient transfection of an expression vector. After the initia...Continue Reading

References

Mar 31, 1999·Endocrinology·Y YoshikoN Maeda
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Aug 20, 2003·The Journal of Biological Chemistry·Su-Li ChengDwight A Towler
Sep 10, 2003·Biochemical and Biophysical Research Communications·Mi-Hye LeeHyun-Mo Ryoo
Jun 24, 2004·Nature Reviews. Genetics·Abigail Tucker, Paul Sharpe
May 14, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·C MorsczeckK H Hoffmann
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Apr 18, 2007·Molecular and Cellular Biology·Yuji YoshikoJane E Aubin
Jul 25, 2008·Journal of Dental Research·S YaoG E Wise
Mar 3, 2009·Clinical Oral Investigations·Christian MorsczeckOliver Driemel
May 4, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Muhammad WasimReinhard Kofler
May 25, 2010·Stem Cells and Development·Michael SaugspierChristian Morsczeck
Jun 21, 2011·Biochemical and Biophysical Research Communications·Sandra Viale-BouroncleChristian Morsczeck
Nov 22, 2011·Differentiation; Research in Biological Diversity·Oliver FelthausChristian Morsczeck
Nov 24, 2011·Stem Cells and Development·Sandra Viale-BouroncleChristian Morsczeck
Dec 16, 2011·Endocrine·Robert S Weinstein
May 29, 2013·Journal of Periodontal Research·O FelthausC Morsczeck

❮ Previous
Next ❯

Citations

May 13, 2014·Cell and Tissue Research·Oliver FelthausChristian Morsczeck
Jan 1, 2016·BMC Biotechnology·Ondine LucaciuBogdan Crisan
Oct 24, 2015·Dental Materials : Official Publication of the Academy of Dental Materials·Eduardo A BortoluzziFranklin R Tay
Oct 18, 2016·International Journal of Implant Dentistry·Martin GosauChristian Morsczeck
Apr 11, 2019·Stem Cells and Development·Jie ZhangChunmiao Jiang
Oct 23, 2019·Stem Cells International·Tao ZhouXin Zhou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.