ZEA exerts toxic effects on reproduction and development by mediating Dio3os in mouse endometrial stromal cells

Journal of Biochemical and Molecular Toxicology
Haiqiang XieYinxue Xu

Abstract

Zearalenone (ZEA) and imprinted long noncoding RNAs (lncRNAs) are both closely related to reproduction and development. However, whether they have connections in regulating reproduction and development is not clear yet. The aim of this research is to investigate their relationship. lncRNA microarray was performed to analyze differentially expressed genes, and real-time quantitative polymerase chain reaction (PCR) was used to verify the accuracy of microarray analysis. Meanwhile, the technologies of rapid amplification of cDNA ends, RNA fluorescence in situ hybridization and bioinformatics were adopted to characterize the selected lncRNA. Analysis of lncRNA microarray showed lncRNAs and messenger RNAs related to reproduction and development were significantly differently expressed, and Dio3os was probably the target lncRNA. Then, the experiment of real-time quantitative PCR verified the accuracy of microarray data. Characterization of Dio3os showed Dio3os, an antisense lncRNA with 2312 bp and 15 open reading frames, was enriched in the cytoplasm. Our findings suggest ZEA probably exerts toxic effects on reproduction and development by mediating Dio3os.

References

Apr 1, 1997·Food Additives and Contaminants·G A MoltoA Pereyra Gonzalez
Apr 9, 1999·The Journal of Clinical Investigation·V A GaltonD L St Germain
Aug 15, 2002·Current Biology : CB·Chen En TsaiAnne C Ferguson-Smith
Sep 1, 2005·Thyroid : Official Journal of the American Thyroid Association·Arturo Hernandez
Sep 6, 2005·Science·S KatayamaUNKNOWN FANTOM Consortium
May 10, 2006·PLoS Genetics·Masaaki FurunoHarukazu Suzuki
Apr 21, 2007·Trends in Genetics : TIG·Florian M PaulerDenise P Barlow
May 13, 2008·Trends in Genetics : TIG·Simao Teixeira da RochaAnne C Ferguson-Smith
Feb 21, 2009·RNA Biology·Paulina A Latos, Denise P Barlow
Apr 21, 2010·Genome Research·Tanmoy MondalChandrasekhar Kanduri
Jul 10, 2010·Toxicon : Official Journal of the International Society on Toxinology·Daniela E MarinDaniela S Tudor
Sep 4, 2010·Plant Physiology·Dharmendra K SinghTimothy W McNellis
Jan 14, 2011·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Magadalena GajęckaMaciej Gajęcki
Dec 12, 2012·Frontiers in Genetics·William H DietzLaura B K Herzing
May 31, 2015·Advanced Drug Delivery Reviews·O KhorkovaC Wahlestedt
Jul 25, 2015·Nature Communications·Tanmoy MondalChandrasekhar Kanduri
Sep 17, 2015·Japanese Journal of Clinical Oncology·Hui-Jin LiWei Chen
Nov 20, 2016·Journal of Biochemical and Molecular Toxicology·Haiqiang XieYinxue Xu

❮ Previous
Next ❯

Citations

May 8, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Liye ZhuWentao Xu
Jul 12, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Li YouKamil Kuca

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.