Zearalenone altered the cytoskeletal structure via ER stress- autophagy- oxidative stress pathway in mouse TM4 Sertoli cells

Scientific Reports
Wang-Long ZhengZong-Ping Liu

Abstract

The aim of this study was to investigate the molecular mechanisms of the destruction of cytoskeletal structure by Zearalenone (ZEA) in mouse-derived TM4 cells. In order to investigate the role of autophagy, oxidative stress and endoplasmic reticulum(ER) stress in the process of destruction of cytoskeletal structure, the effects of ZEA on the cell viability, cytoskeletal structure, autophagy, oxidative stress, ER stress, MAPK and PI3K- AKT- mTOR signaling pathways were studied. The data demonstrated that ZEA damaged the cytoskeletal structure through the induction of autophagy that leads to the alteration of cytoskeletal structure via elevated oxidative stress. Our results further showed that the autophagy was stimulated by ZEA through PI3K-AKT-mTOR and MAPK signaling pathways in TM4 cells. In addition, ZEA also induced the ER stress which was involved in the induction of the autophagy through inhibiting the ERK signal pathway to suppress the phosphorylation of mTOR. ER stress was involved in the damage of cytoskeletal structure through induction of autophagy by producing ROS. Taken together, this study revealed that ZEA altered the cytoskeletal structure via oxidative stress - autophagy- ER stress pathway in mouse TM4 Sertoli c...Continue Reading

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Citations

Nov 23, 2018·Journal of Food Science·Ankita RaiAnurag Tripathi
Apr 3, 2020·Toxins·Karolina KowalskaAgnieszka Wanda Piastowska-Ciesielska
May 5, 2018·Toxins·Wanglong ZhengZongping Liu
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Apr 4, 2021·Ecotoxicology and Environmental Safety·Weiqi SunZhijun Zhou
Jun 23, 2021·Environmental Science and Pollution Research International·Li CaoXichun Wang
Sep 4, 2021·Ecotoxicology and Environmental Safety·Xu YangYanfei Li
Nov 25, 2021·The Journal of International Medical Research·Xiaodong LuoXiaojing Dong

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Methods Mentioned

BETA
fluorescence microscopy
RAP
flow cytometry
protein folding
electrophoresis
Transfection

Software Mentioned

SPSS
Image Lab

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