Zearalenone Inhibits Rat and Human 11β-Hydroxysteroid Dehydrogenase Type 2

BioMed Research International
Linxi LiRen-Shan Ge

Abstract

Zearalenone is a mycotoxin produced by Fusarium spp. 11β-Hydroxysteroid dehydrogenases, isoforms 1 (HSD11B1) and 2 (HSD11B2), have been demonstrated to be the regulators of the local level of active glucocorticoid, which has a broad range of physiological actions. In the present study, the potency of zearalenone was tested for the inhibition of HSD11B1 and HSD11B2 in rat and human tissues. Zearalenone showed potent inhibition of HSD11B2 with the half-maximal inhibitory concentration (IC50) calculated at 49.63 and 32.22 μM for the rat and human, respectively. Results showed that zearalenone competitively inhibited HSD11B2 when a steroid substrate was used. However, it served as an uncompetitive inhibitory factor when the cofactor NAD(+) was used. In contrast, the potency of zearalenone to inhibit both rat and human HSD11B1 was diminished, with the concentration of 100 μM causing almost no inhibitory effect on the isoform. In conclusion, we observed that zearalenone is a selective inhibitor of HSD11B2, implying that this agent may cause excessive glucocorticoid action in local tissues such as kidney and placentas.

References

May 1, 1992·Journal of Animal Science·M A Diekman, M L Green
Sep 1, 1987·Regulatory Toxicology and Pharmacology : RTP·T Kuiper-GoodmanH Watanabe
Mar 1, 1985·Journal of Steroid Biochemistry·V Lakshmi, C Monder
Nov 1, 1994·Molecular and Cellular Endocrinology·A L AlbistonZ S Krozowski
Dec 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·J R SecklR W Brown
Jan 10, 2003·The Journal of Clinical Endocrinology and Metabolism·Eero KajantieSture Andersson
May 23, 2007·Nature Clinical Practice. Endocrinology & Metabolism·Jonathan R Seckl, Megan C Holmes
Mar 31, 2009·International Journal of Molecular Sciences·Fiorenza Minervini, Maria Elena Dell'Aquila
Jul 19, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Daniela E MarinLoredana Calin
Apr 16, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Martin HillAntonín Pařízek
Jun 18, 2014·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Ali S AlzahraniNaffaa Alharbi
Feb 17, 2015·Chemico-biological Interactions·Silvana Peterini BoeiraAna Flávia Furian
Mar 26, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Robert H Oakley, John A Cidlowski

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Citations

May 21, 2016·International Journal of Endocrinology·Katarzyna KosickaFranciszek K Główka
Apr 4, 2017·Environmental Toxicology and Pharmacology·Chunchun ZhouQuanxu Chen
Apr 25, 2020·Archives of Toxicology·Erica BuosoEmanuela Corsini

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BETA
Protein Assay

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GraphPad

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