Zebrafish and Medaka: new model organisms for modern biomedical research

Journal of Biomedical Science
Cheng-Yung LinHuai-Jen Tsai

Abstract

Although they are primitive vertebrates, zebrafish (Danio rerio) and medaka (Oryzias latipes) have surpassed other animals as the most used model organisms based on their many advantages. Studies on gene expression patterns, regulatory cis-elements identification, and gene functions can be facilitated by using zebrafish embryos via a number of techniques, including transgenesis, in vivo transient assay, overexpression by injection of mRNAs, knockdown by injection of morpholino oligonucleotides, knockout and gene editing by CRISPR/Cas9 system and mutagenesis. In addition, transgenic lines of model fish harboring a tissue-specific reporter have become a powerful tool for the study of biological sciences, since it is possible to visualize the dynamic expression of a specific gene in the transparent embryos. In particular, some transgenic fish lines and mutants display defective phenotypes similar to those of human diseases. Therefore, a wide variety of fish model not only sheds light on the molecular mechanisms underlying disease pathogenesis in vivo but also provides a living platform for high-throughput screening of drug candidates. Interestingly, transgenic model fish lines can also be applied as biosensors to detect environmen...Continue Reading

References

Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·D SmallA M Gewirtz
Dec 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·Z Q MaA Maggi
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J R JessenS Lin
Mar 12, 1999·Mechanisms of Development·N Scheer, J A Campos-Ortega
Apr 18, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C D HsiaoH J Tsai
May 30, 2001·Molecular and Cellular Endocrinology·H HuangS Lin
Nov 24, 2001·Mutation Research·Kimiko AmanumaYasunobu Aoki
Jun 28, 2002·Cancer Cell·James F AmatrudaLeonard I Zon
Jul 20, 2002·Mechanisms of Development·Anne Vatland Krøvel, Lisbeth Charlotte Olsen
Jul 27, 2002·Annual Review of Genomics and Human Genetics·Jordan T Shin, Mark C Fishman
Jun 20, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Guor Mour HerJen-Leih Wu
Aug 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Angel RayaJuan Carlos Izpisúa-Belmonte
Sep 2, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Chiu-Ju HuangHuai-Jen Tsai
Sep 2, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yau-Hung ChenHuai-Jen Tsai
Nov 7, 2003·Development·Xiaodong ShuJau-Nian Chen
Jul 9, 2004·Developmental Cell·Koichi KawakamiMasayoshi Mishina
Apr 14, 2005·Blood·Gerhard J WeberLeonard I Zon
Sep 6, 2005·Experimental Hematology·Nelson Hsia, Leonard I Zon
Nov 19, 2005·Journal of Biomedical Optics·Michael LieblingMary E Dickinson
Jan 3, 2006·Developmental Biology·Hung-Chieh LeeHuai-Jen Tsai
Feb 21, 2006·American Journal of Physiology. Heart and Circulatory Physiology·David J MilanCalum A MacRae
May 6, 2006·Science·Arian S ForouharMorteza Gharib
Jun 1, 2006·Nature Genetics·Nikolaus Rajewsky
Jan 4, 2007·BMC Developmental Biology·Yau-Hung ChenHuai-Jen Tsai
Jun 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Rima ArnaoutNeil C Chi
Feb 26, 2008·Cell·Stuart H Orkin, Leonard I Zon
Feb 26, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yun-Hsin WangYau-Hung Chen
Apr 1, 2008·Oncogene·K Stoletov, R Klemke
May 29, 2008·Molecular Cancer Research : MCR·Harma Feitsma, Edwin Cuppen
Dec 20, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hsing-Yen HuangHuai-Jen Tsai
Jan 28, 2009·Nature Chemical Biology·Jing-Ruey J YehRandall T Peterson

❮ Previous
Next ❯

Citations

May 18, 2016·International Journal of Molecular Sciences·Atsuo KawaharaKiyohito Taimatsu
Mar 23, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Andreas SchüttlerWibke Busch
Jan 6, 2017·ILAR Journal·Kevin Strange
Dec 22, 2017·British Journal of Haematology·Kasem KulkeawDaisuke Sugiyama
Aug 17, 2020·International Journal of Molecular Sciences·Marián MerinoAna Virginia Sánchez-Sánchez
Aug 31, 2016·The Biochemical Journal·Amy E Campbell, Daimark Bennett
Jul 14, 2017·The Journal of Cell Biology·Paola KuriMaria Leptin
Oct 13, 2018·Toxicology Research·Longlue JiaJiangfeng Guo
Jan 4, 2017·Developmental Medicine and Child Neurology·Michael C FaheyMichael C Kruer
Nov 20, 2016·Bioscience, Biotechnology, and Biochemistry·Yuki TakadaKiyotaka Hitomi
Aug 3, 2019·Scientific Reports·Marta F RiescoVanesa Robles
Aug 31, 2019·Metallomics : Integrated Biometal Science·Man Long Kwok, King Ming Chan
Oct 3, 2020·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Katsuhiro KonnoDaisuke Sugiyama
Dec 22, 2020·International Journal of Radiation Biology·Yeni Natalia C Perez-GelvezCarl W Bergmann
Feb 17, 2021·Bioscience, Biotechnology, and Biochemistry·Yuko WatanabeKiyotaka Hitomi
Sep 18, 2020·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Pai LiuWen-Fei Dong
Jul 14, 2019·Journal of Environmental Management·Loraine Soto-VázquezFrancisco M Márquez-Linares
Nov 13, 2020·Physiological Reviews·Yoshitaka NagahamaMasaru Nakamura
Jun 3, 2021·Pharmaceuticals·Hung-Chieh LeeHuai-Jen Tsai
Jul 3, 2021·Pharmaceuticals·Siripat ChaichitMotoyuki Itoh
Feb 11, 2020·Chemical Science·Xiujuan ShiBen Zhong Tang
Jul 27, 2021·IScience·Jose Fernando López-OlmedaNicholas S Foulkes
Aug 24, 2021·Nanoscale Research Letters·Suraiya Saleem, Rajaretinam Rajesh Kannan
Aug 26, 2020·Environmental Science & Technology·Jiezhang MoDr Frauke Seemann
Oct 23, 2021·Frontiers in Neuroendocrinology·Paula G VissioJulieta E Sallemi

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
targeted
genetic knockout
Pull-down
xenograft
chemical
Biosensor

Software Mentioned

TIME

Related Concepts

Related Feeds

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.