Zebrafish androgen receptor is required for spermatogenesis and maintenance of ovarian function

Oncotarget
Guangqing YuWuhan Xiao

Abstract

The androgen receptor (AR) is a nuclear receptor protein family member and inducible transcription factor that modulates androgen target gene expression. Studies using a mouse model confirmed the need for ar in reproductive development, particularly spermatogenesis. Here, we investigated the role of ar in zebrafish using CRISPR/Cas9 gene targeting technology. Targeted disruption of ar in zebrafish increases the number of female offspring and increases offspring weight. In addition, ar-null male zebrafish have female secondary sex characteristics. More importantly, targeted disruption of ar in zebrafish causes male infertility via defective spermatogenesis and female premature ovarian failure during growth. Mechanistic assays suggest that these effects are caused by fewer proliferated cells and more apoptotic cells in ar-null testes. Moreover, genes involved in reproductive development, estradiol induction and hormone synthesis were dys-regulated in testes and ovaries and the reproductive-endocrine axis was disordered. Our data thus suggest that the zebrafish ar is required for spermatogenesis and maintenance of ovarian function, which confirms evolutionarily conserved functions of ar in vertebrates, as well as indicates that ar...Continue Reading

References

Feb 27, 1992·The New England Journal of Medicine·J E Griffin
Aug 1, 1989·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·R BalducciV Toscano
Feb 20, 1970·Science·C W BardinA J Stanley
Sep 19, 1970·Nature·M F Lyon, S G Hawkes
Jun 1, 1995·Endocrine Reviews·C A QuigleyF S French
Jul 27, 1999·The Journal of Steroid Biochemistry and Molecular Biology·M J McPhaul
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·J W Thornton
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Shuyuan YehMin-Chi Hung
Dec 14, 2002·Biochemical and Biophysical Research Communications·Takashi SatoShigeaki Kato
Aug 29, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Takahiro MatsumotoShigeaki Kato
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Karel De GendtGuido Verhoeven
Jan 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Takashi SatoShigeaki Kato
Apr 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chawnshang ChangShuyuan Yeh
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yueh-Chiang HuChawnshang Chang
Dec 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hiroko ShiinaShigeaki Kato
Apr 7, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Katrien VenkenDirk Vanderschueren
Nov 2, 2006·Hormone Research·Sabine E Hannema, Ieuan A Hughes
Dec 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Meng-Yin TsaiChawnshang Chang
Oct 19, 2007·Biology of Reproduction·Mohammad Sorowar HossainLaszlo Orban
Apr 9, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Helen E MacLeanJeffrey D Zajac
May 13, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·P P de WaalJ Bogerd
Sep 26, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Daniel A GorelickMarnie E Halpern
May 9, 2009·Molecular and Cellular Endocrinology·Laszlo OrbanPer-Erik Olsson
Jun 9, 2009·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Jin-Xia PengJian-Fang Gui
Nov 13, 2009·The Journal of Clinical Investigation·Jiann-Jyh LaiChawnshang Chang

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Citations

Sep 26, 2020·Archives of Toxicology·Alex C KingArmin K Zenker
Jul 16, 2020·Archives of Environmental Contamination and Toxicology·John B ChiariCourtney L McGinnis
Jan 20, 2020·Cellular and Molecular Life Sciences : CMLS·Minghui LiDeshou Wang
Oct 1, 2020·Computational and Structural Biotechnology Journal·Mengling Ye, Ye Chen
Dec 23, 2021·Cellular and Molecular Life Sciences : CMLS·Devora Aharon, Florence L Marlow

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Methods Mentioned

BETA
PCR
dissection
enzyme-linked immunosorbent assay

Key Resources (RRID) Mentioned

AB_2631966
AB_1009478

Software Mentioned

GraphPad
GraphPad Prism
Excel

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