Zebrafish as an appealing model for optogenetic studies

Progress in Brain Research
Joshua SimmichEthan K Scott

Abstract

Optogenetics, the use of light-based protein tools, has begun to revolutionize biological research. The approach has proven especially useful in the nervous system, where light has been used both to detect and to manipulate activity in targeted neurons. Optogenetic tools have been deployed in systems ranging from cultured cells to primates, with each offering a particular combination of advantages and drawbacks. In this chapter, we provide an overview of optogenetics in zebrafish. Two of the greatest attributes of the zebrafish model system are external fertilization and transparency in early life stages. Combined, these allow researchers to observe the internal structures of developing zebrafish embryos and larvae without dissections or other interference. This transparency, combined with the animals' small size, simple husbandry, and similarity to mammals in many structures and processes, has made zebrafish a particularly popular model system in developmental biology. The easy optical access also dovetails with optogenetic tools, allowing their use in intact, developing, and behaving animals. This means that optogenetic studies in embryonic and larval zebrafish can be carried out in a high-throughput fashion with relatively s...Continue Reading

Citations

Feb 14, 2017·Reproductive Toxicology·Muhammad Ibrahim, Michael K Richardson
Dec 4, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Idan ElbazLior Appelbaum
Jun 26, 2020·Frontiers in Cell and Developmental Biology·Adam Varady, Martin Distel
Oct 21, 2020·Scientific Reports·Ilenia MeloniCaroline Murawski
Mar 4, 2021·Current Biology : CB·Rebecca E PoulsenEthan K Scott

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