Zebrafish foxc1a plays a crucial role in early somitogenesis by restricting the expression of aldh1a2 directly.

The Journal of Biological Chemistry
Jingyun LiQing-Shun Zhao

Abstract

Foxc1a is a member of the forkhead transcription factors. It plays an essential role in zebrafish somitogenesis. However, little is known about the molecular mechanisms underlying its controlling somitogenesis. To uncover how foxc1a regulates zebrafish somitogenesis, we generated foxc1a knock-out zebrafish using TALEN (transcription activator-like effector nuclease) technology. The foxc1a null embryos exhibited defective somites at early development. Analyses on the expressions of the key genes that control processes of somitogenesis revealed that foxc1a controlled early somitogenesis by regulating the expression of myod1. In the somites of foxc1a knock-out embryos, expressions of fgf8a and deltaC were abolished, whereas the expression of aldh1a2 (responsible for providing retinoic acid signaling) was significantly increased. Once the increased retinoic acid level in the foxc1a null embryos was reduced by knocking down aldh1a2, the reduced expression of myod1 was partially rescued by resuming expressions of fgf8a and deltaC in the somites of the mutant embryos. Moreover, a chromatin immunoprecipitation assay on zebrafish embryos revealed that Foxc1a bound aldh1a2 promoter directly. On the other hand, neither knocking down fgf8a...Continue Reading

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Citations

Oct 15, 2018·Journal of Cellular Physiology·Jingyun LiXiaohua Dong
May 3, 2019·Journal of Cellular and Molecular Medicine·Chong ChenJianxiong Shen
Jul 29, 2020·Human Molecular Genetics·Jesús-José Ferre-FernándezElena V Semina
Feb 10, 2021·Journal of the Royal Society, Interface·Supriya BajpaiMandar M Inamdar
Feb 4, 2021·Genes·Paul W ChrystalAndrew J Waskiewicz
Jul 28, 2021·Annual Review of Genetics·Gabrielius Jakutis, Didier Y R Stainier

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