Zebrafish mutants with disrupted early T-cell and thymus development identified in early pressure screen.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
N S TredeLeonard I Zon

Abstract

Generation of mature T lymphocytes requires an intact hematopoietic stem cell compartment and functional thymic epithelium. We used the zebrafish (Danio rerio) to isolate mutations that affect the earliest steps in T lymphopoiesis and thymic organogenesis. Here we describe the results of a genetic screen in which gynogenetic diploid offspring from heterozygous females were analyzed by whole-mount in situ hybridization for the expression of rag-1. To assess immediately if a global defect in hematopoiesis resulted in the mutant phenotype, alpha-embryonic globin expression was simultaneously assayed for multilineage defects. In this report, we present the results obtained with this strategy and show representative mutant phenotypes affecting early steps in T-cell development and/or thymic epithelial cell development. We discuss the advantage of this strategy and the general usefulness of the zebrafish as a model system for vertebrate lymphopoiesis and thymic organogenesis.

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Citations

Oct 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Narges AghaallaeiThomas Boehm
Dec 31, 2011·Genome Medicine·Corey S MartinLeonard I Zon
Jan 10, 2012·Disease Models & Mechanisms·Stephen A Renshaw, Nikolaus S Trede
Oct 29, 2010·Genetics·Bradley L DemarestNikolaus S Trede
Jul 2, 2009·Journal of Visualized Experiments : JoVE·Charline WalkerCecilia Moens
Jun 19, 2012·Annual Review of Genomics and Human Genetics·Thomas BoehmIsabell Hess
Oct 8, 2015·Blood Reviews·Xiangguo ShiAnskar Y H Leung
May 13, 2014·Experimental Hematology·Kelli J Carroll, Trista E North
Sep 15, 2012·Current Biology : CB·Thomas Boehm
Jun 5, 2012·Developmental Biology·Sarah A HutchinsonNikolaus S Trede
Feb 27, 2014·Biotechnology & Genetic Engineering Reviews·Mian Yahya MushtaqHye Kyong Kim
Feb 13, 2018·Wiley Interdisciplinary Reviews. Developmental Biology·Aniket V GoreBrant M Weinstein
May 29, 2018·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Triveni Menon, Sreelaja Nair
Aug 29, 2019·International Journal of Molecular Sciences·Baubak BajoghliNarges Aghaallaei
Apr 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Oliver W StockhammerAnnemarie H Meijer

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