Zebrafish prdm12b acts independently of nkx6.1 repression to promote eng1b expression in the neural tube p1 domain

Neural Development
Ozge YildizCharles G Sagerström

Abstract

Functioning of the adult nervous system depends on the establishment of neural circuits during embryogenesis. In vertebrates, neurons that make up motor circuits form in distinct domains along the dorsoventral axis of the neural tube. Each domain is characterized by a unique combination of transcription factors (TFs) that promote a specific fate, while repressing fates of adjacent domains. The prdm12 TF is required for the expression of eng1b and the generation of V1 interneurons in the p1 domain, but the details of its function remain unclear. We used CRISPR/Cas9 to generate the first germline mutants for prdm12 and employed this resource, together with classical luciferase reporter assays and co-immunoprecipitation experiments, to study prdm12b function in zebrafish. We also generated germline mutants for bhlhe22 and nkx6.1 to examine how these TFs act with prdm12b to control p1 formation. We find that prdm12b mutants lack eng1b expression in the p1 domain and also possess an abnormal touch-evoked escape response. Using luciferase reporter assays, we demonstrate that Prdm12b acts as a transcriptional repressor. We also show that the Bhlhe22 TF binds via the Prdm12b zinc finger domain to form a complex. However, bhlhe22 mutant...Continue Reading

References

Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Apr 15, 1999·Annual Review of Neuroscience·K J Lee, T M Jessell
Nov 21, 2000·Current Opinion in Neurobiology·T M Jessell, J R Sanes
Feb 13, 2001·Progress in Neurobiology·R C EatonM B Foreman
May 1, 2001·The Journal of Biological Chemistry·S PalmerC Bartholomew
Jul 3, 2002·Nature Reviews. Neuroscience·Nicolas BertrandFrançois Guillemot
Feb 27, 2004·Nature Immunology·Ildikó GyoryKenneth L Wright
Mar 31, 2004·The Journal of Cell Biology·Alexandra ChittkaMichael Sendtner
Jun 25, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shin-ichi HigashijimaJoseph R Fetcho
Jun 7, 2005·Developmental Biology·Leigh Wilson, Malcolm Maden
Jun 21, 2005·Biochemical and Biophysical Research Communications·Céline DerunesKathryn R Ely
Oct 29, 2005·The Journal of Comparative Neurology·Francisco J AlvarezMartyn Goulding
Mar 10, 2006·Nature·Simon GosgnachMartyn Goulding
Mar 16, 2006·Molecular and Cellular Biology·Christopher A DavisEric N Olson
Jun 16, 2007·Developmental Biology·Fatma O KokHoward I Sirotkin
Jul 20, 2007·Molecular and Cellular Biology·Zhijun DuanMarshall S Horwitz
May 17, 2008·Genes & Development·Shingo KajimuraBruce M Spiegelman
Feb 27, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Denise A Zannino, Bruce Appel
Jun 23, 2009·Nature Reviews. Neuroscience·Martyn Goulding
Aug 4, 2009·Biochemical and Biophysical Research Communications·Gwang Hyeon EomSang-Beom Seo
Oct 27, 2011·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Cathrine K FogAnders H Lund
Jan 26, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Denise A ZanninoBruce Appel

❮ Previous
Next ❯

Citations

Jul 23, 2020·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Young Sang KwonJong-Su Seo
Mar 29, 2021·The FEBS Journal·Federico Di TullioErnesto Guccione

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
in vitro transcription
transfections
transfection
co-IP
genotyping
immunoprecipitation
co-immunoprecipitation

Software Mentioned

CHOPCHOP
Adobe Photoshop

Related Concepts

Related Feeds

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.