Zebrafish xenografts as a fast screening platform for bevacizumab cancer therapy.

Communications Biology
Cátia Rebelo de AlmeidaRita Fior

Abstract

Despite promising preclinical results, average response rates to anti-VEGF therapies, such as bevacizumab, are reduced for most cancers, while incurring in remarkable costs and side effects. Currently, there are no biomarkers available to select patients that can benefit from this therapy. Depending on the individual tumor, anti-VEGF therapies can either block or promote metastasis. In this context, an assay able to predict individual responses prior to treatment, including the impact on metastasis would prove of great value to guide treatment options. Here we show that zebrafish xenografts are able to reveal different responses to bevacizumab in just 4 days, evaluating not only individual tumor responses but also the impact on angiogenesis and micrometastasis. Importantly, we perform proof-of-concept experiments where clinical responses in patients were compared with their matching zebrafish Patient-Derived Xenografts - zAvatars, opening the possibility of using the zebrafish model to screen bevacizumab therapy in a personalized manner.

References

Sep 27, 2000·Neoplasia : an International Journal for Oncology Research·J L RubensteinM A Shuman
Dec 13, 2000·The Journal of Pathology·R E HewittM Tsoskas
Aug 9, 2002·Developmental Biology·Nathan D Lawson, Brant M Weinstein
Nov 4, 2004·Current Topics in Developmental Biology·Katsutoshi Goishi, Michael Klagsbrun
Jul 18, 2006·Current Medicinal Chemistry·Girolamo RanieriDomenico Ribatti
Nov 14, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Ferry A L M Eskens, Jaap Verweij
Apr 6, 2007·Cancer Research·Stefania NicoliMarco Presta
Mar 29, 2008·Cell Stem Cell·Richard Mark WhiteLeonard I Zon
Jul 25, 2008·Nature Reviews. Cancer·Gabriele Bergers, Douglas Hanahan
Mar 25, 2009·Nature Reviews. Cancer·Don X NguyenJoan Massagué
Apr 30, 2009·BMC Cancer·Ines J MarquesChristoph P Bagowski
Jun 10, 2010·Journal of Cell Science·Konstantin StoletovRichard Klemke
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Olivier KeunenSimone P Niclou
Mar 3, 2011·Nature Reviews. Clinical Oncology·John M L Ebos, Robert S Kerbel
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Jun 2, 2011·Nature Reviews. Drug Discovery·Peter Carmeliet, Rakesh K Jain
Jul 27, 2011·Therapeutic Advances in Medical Oncology·Shari B GoldfarbMaura N Dickler
Oct 18, 2011·Cell·Scott Valastyan, Robert A Weinberg
Oct 21, 2011·Current Oncology Reports·Alberto J MonteroCharles Vogel
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Nov 23, 2013·Nature Reviews. Cancer·Hira Lal Goel, Arthur M Mercurio
Jul 6, 2014·Investigational New Drugs·Yingmiao LiuAndrew B Nixon
Jan 9, 2015·The Oncologist·Alba A BrandesEnrico Franceschi
Aug 25, 2017·Proceedings of the National Academy of Sciences of the United States of America·Rita FiorMiguel Godinho Ferreira
Nov 11, 2017·Nucleic Acids Research·David S WishartMichael Wilson
Jul 23, 2018·Annals of Oncology : Official Journal of the European Society for Medical Oncology·F CardosoE P Winer

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Citations

Feb 20, 2021·Briefings in Functional Genomics·Veronica Bergo, Eirini Trompouki
Mar 2, 2021·Frontiers in Cell and Developmental Biology·Xingyu ChenRenbing Jia
Jun 3, 2021·Journal of Experimental & Clinical Cancer Research : CR·Giulia RizzoStefania Vetrano
Jun 13, 2021·Nature Reviews. Drug Discovery·E Elizabeth PattonDavid M Langenau
Jun 25, 2021·Scientific Reports·Gautier FollainJacky G Goetz
Jan 22, 2022·SLAS Technology·Jason J OtterstromYael Paran

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Methods Mentioned

BETA
Xenografts
confocal microscopy
xenograft
biopsy

Software Mentioned

GraphPad Prism
ImageJ
Imaris
Fiji

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