Zein protein interactions, rather than the asymmetric distribution of zein mRNAs on endoplasmic reticulum membranes, influence protein body formation in maize endosperm

The Plant Cell
Cheol Soo KimB A Larkins

Abstract

Prolamin-containing protein bodies in maize endosperm are composed of four different polypeptides, the alpha-, beta-, gamma-, and delta-zeins. The spatial organization of zeins within the protein body, as well as interactions between them, suggests that the localized synthesis of gamma-zeins could initiate and target protein body formation at specific regions of the rough endoplasmic reticulum. To investigate this possibility, we analyzed the distribution of mRNAs encoding the 22-kD alpha-zein and the 27-kD gamma-zein proteins on cisternal and protein body rough endoplasmic reticulum membranes. In situ hybridization revealed similar frequencies of the mRNAs in both regions of the endoplasmic reticulum, indicating that the transcripts are distributed more or less randomly. This finding implies that zein protein interactions determine protein body assembly. To address this question, we expressed cDNAs encoding alpha-, beta-, gamma-, and delta-zeins in the yeast two-hybrid system. We found strong interactions among the 50-, 27-, and 16-kD gamma-zeins and the 15-kD beta-zein, consistent with their colocalization in developing protein bodies. Interactions between the 19- and 22-kD alpha-zeins were relatively weak, although each of t...Continue Reading

References

Dec 1, 1992·The Plant Cell·D E BostwickG A Thompson
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M P Yaffe, G Schatz
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Apr 21, 1995·Cell·D St Johnston
Jan 1, 1995·Plant Physiology·C F Chui, S C Falco
Oct 1, 1993·The Plant Cell·M A Lopes, B A Larkins
Nov 1, 1995·The Plant Journal : for Cell and Molecular Biology·J SheenD W Galbraith
Oct 1, 1996·Plant Molecular Biology·M Bar-PeledN V Raikhel
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·C E ColemanB A Larkins
Nov 5, 1997·The Journal of Biological Chemistry·D A ShaywitzC A Kaiser
Apr 24, 1999·The Plant Cell·K Keegstra, K Cline

❮ Previous
Next ❯

Citations

Jun 22, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·H K C LaidlawI D Godwin
Sep 25, 2012·Plant Cell Reports·Mathilde Francin-AllamiYves Popineau
Feb 24, 2005·Planta·Jennifer J RandallJohn D Kemp
Feb 26, 2013·Planta·Wei ZhangJoachim Messing
Mar 31, 2005·Trends in Genetics : TIG·Bryan C Gibbon, Brian A Larkins
Oct 24, 2002·Current Opinion in Plant Biology·Thomas W Okita, Sang Bong Choi
Jun 6, 2009·Journal of Experimental Botany·Lorena MoellerKan Wang
Apr 28, 2011·Plant & Cell Physiology·Ai NagamineToshihiro Kumamaru
Oct 22, 2005·Exercise and Sport Sciences Reviews·David J Dyck
Mar 23, 2006·Pancreas·Eija TukiainenHeikki Repo
Mar 11, 2010·American Journal of Therapeutics·Christian FouilliouxValmore Bermúdez
Aug 19, 2007·American Journal of Cardiovascular Drugs : Drugs, Devices, and Other Interventions·Benoit DroletPaul Poirier
Oct 6, 2014·Current Opinion in Plant Biology·Li Tian, Thomas W Okita
Jul 23, 2014·Biotechnology Letters·Maite SabalzaTeresa Capell
Dec 27, 2015·Plant Science : an International Journal of Experimental Plant Biology·Mustapha BenmoussaBruce R Hamaker
Jun 21, 2011·Plant Science : an International Journal of Experimental Plant Biology·Tomokazu UshijimaToshihiro Kumamaru
May 10, 2008·Biochimica Et Biophysica Acta·Vanessa CabraRoberto Arreguin-Espinosa
May 16, 2008·Journal of Separation Science·Guillaume L ErnyAlejandro Cifuentes
Oct 3, 2006·The Plant Journal : for Cell and Molecular Biology·Cheol Soo KimRudolf Jung
Nov 13, 2007·Biopolymers·Tatiana C BicudoLuiz A Colnago
Jun 27, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Philip W Becraft, Jose Gutierrez-Marcos
Jul 8, 2014·Frontiers in Plant Science·David R Holding
Sep 1, 2005·Analytical and Bioanalytical Chemistry·Tatiana C BicudoLuiz A Colnago
Dec 8, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Andrew J CroftsHikaru Satoh
Jan 4, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chaobin Li, Rentao Song
Oct 22, 2017·Genetics·Jose Planta, Joachim Messing
Feb 24, 2011·The Plant Cell·Francisca C ReyesMarisa S Otegui

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.