Zeolin is a recombinant storage protein with different solubility and stability properties according to its localization in the endoplasmic reticulum or in the chloroplast

Journal of Biotechnology
Michele BellucciSergio Arcioni

Abstract

Several strategies have been exploited to maximize heterologous protein accumulation in the plant cell. Recently, it has been shown that a portion of a maize prolamin storage protein, gamma-zein, can be used for the high accumulation of a recombinant protein in novel endoplasmic reticulum (ER)-derived protein bodies of vegetative tissues. In this study, we investigate whether this protein can be expressed in the chloroplast. Our long-term purpose is to use zeolin to produce value-added proteins by fusing these polypeptides with its gamma-zein portion and targeting the recombinant proteins to the ER or to the chloroplast. We show here that zeolin accumulates in the chloroplast to lower levels than in the ER and its stability is compromised by chloroplast proteolytic activity. Co-localization of zeolin and the ER chaperone BiP in the chloroplast does not have a beneficial effect on zeolin accumulation. In this organelle, zeolin is not stored in protein bodies, nor do zeolin polypeptides seem to be linked by inter-chain disulfide bonds, which are usually formed by the six cysteine of the gamma-zein portion, indicating abnormal folding of the recombinant protein. Therefore, it is concluded that to accumulate zeolin in the chloropla...Continue Reading

References

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Citations

Jan 30, 2013·Molecular Biotechnology·Stefan R Schmidt
Aug 9, 2012·Plant Physiology·Francesca De MarchisMichele Bellucci
Aug 18, 2010·Plant Molecular Biology·Francesca De MarchisMichele Bellucci
Jan 19, 2016·Frontiers in Plant Science·Sean MileticRima Menassa
Dec 1, 2015·The Plant Journal : for Cell and Molecular Biology·Xiaochen YangDiane C Bassham
Jun 28, 2013·International Journal of Molecular Sciences·Goretti Virgili-LópezAlessandro Vitale
Feb 2, 2018·Critical Reviews in Food Science and Nutrition·Domancar Orona-TamayoOctavio Paredes-López
Jan 14, 2011·Journal of Agricultural and Food Chemistry·Hari B KrishnanHarold N Trick

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