PMID: 12782034Jun 5, 2003Paper

Zeolites as new chromatographic carriers for proteins--easy recovery of proteins adsorbed on zeolites by polyethylene glycol

Analytical Biochemistry
Hiroyuki ChikuKengo Sakaguchi

Abstract

Zeolites are able to adsorb proteins on their surface and might be suitable as a new type of chromatographic carrier material for proteins and for their conjugates (Matsui et al., Chem. Eur. J. 7 (2001) 1555-1560). Interestingly, maximum adsorption was observed at the isoelectric point (pI) of each protein. The current study was performed to investigate the desorption of proteins from the zeolites at pI. Proteins adsorbed to zeolites could be desorbed at pI by polyethylene glycol (PEG), but not by conventional eluents. The eluted proteins still retained their activities. The zeolite Na-BEA was an especially good composite for desorption by PEG. Using this method for the adsorption and desorption of proteins at pI, we succeeded in separating various proteins. The application of zeolites to biochemistry and biotechnology is also discussed.

References

Aug 16, 2000·The Journal of Biological Chemistry·Y MizushinaK Sakaguchi
May 2, 2001·Chemistry : a European Journal·M MatsuiK Sakaguchi
Jul 27, 2002·Journal of Chromatography. a·Ming LiZhiguo Su
May 17, 1999·Angewandte Chemie·Yoshikatsu AkiyamaKengo Sakaguchi

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Citations

Jan 20, 2005·Applied Microbiology and Biotechnology·Kengo SakaguchiFujio Mizukami
Mar 29, 2013·Parasitology Research·Anwar SunnaPeter L Bergquist
May 23, 2009·Biotechnology Progress·Takayuki Y NaraTatsuo Tsunoda
Feb 19, 2009·Biotechnology Progress·Hideaki TogashiFujio Mizukami
Nov 10, 2006·Journal of Chromatography. a·R WijntjeP J T Bussmann
Apr 10, 2018·Biomaterials Science·Lucie BacakovaIvan Jirka
Dec 1, 2005·Analytical Biochemistry·Hiroyuki ChikuKengo Sakaguchi
Nov 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anna Bodzon-KulakowskaJerzy Silberring
Aug 1, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Michael J Lucas, Benjamin K Keitz

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