Zeptomole Biosensing of DNA with Flexible Selectivity Based on Acoustic Levitation of a Single Microsphere Binding Gold Nanoparticles by Hybridization

ACS Sensors
Akihisa MiyagawaTetsuo Okada

Abstract

A novel scheme for DNA sensing at the zeptomole level is presented, based on the levitation of a single microsphere in a combined acoustic-gravitational (CAG) field. The levitation of a microsphere in the field is predominantly determined by its density. Capture and reporter probe DNAs are anchored on poly(methyl methacrylate) microsphere (PMMA) and gold nanoparticles (AuNPs), respectively, and a target DNA induces the binding of AuNPs on PMMA. This interparticle sandwich DNA-hybridization induces density increase in PMMA, which is detected as a shift in the levitation coordinate in the CAG field. The reporter DNAs are designed based on base-pair (bp) number selectivity, which is evaluated using direct interparticle hybridization between DNA-bound PMMA and complementary DNA-anchored AuNPs. Interestingly, the bp-number selectivity can be enlarged by lowering the reactant concentrations. Thus, the threshold bp, at which no density change is detected, can be adjusted by controlling the reactant concentrations. This strategy is extended to the sensing of HIV-2 DNA and single nucleotide polymorphism (SNP) detection of the KRAS gene by sandwich hybridization. In SNP detection, the present method selectively distinguishes wild-type DN...Continue Reading

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Citations

Sep 15, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Akihisa Miyagawa, Tetsuo Okada

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