Zeptomole electrochemical detection of metallothioneins.

PloS One
Vojtech AdamRene Kizek

Abstract

Thiol-rich peptides and proteins possess a large number of biological activities and may serve as markers for numerous health problems including cancer. Metallothionein (MT), a small molecular mass protein rich in cysteine, may be considered as one of the promising tumour markers. The aim of this paper was to employ chronopotentiometric stripping analysis (CPSA) for highly sensitive detection of MT. In this study, we used adsorptive transfer stripping technique coupled with CPSA for detection of cysteine, glutathione oxidized and reduced, phytochelatin, bovine serum albumin, and metallothionein. Under the optimal conditions, we were able to estimate detection limits down to tens of fg per ml. Further, this method was applied to detect metallothioneins in blood serum obtained from patients with breast cancer and in neuroblastoma cells resistant and sensitive to cisplatin in order to show the possible role of metallothioneins in carcinogenesis. It was found that MT level in blood serum was almost twice higher as compared to the level determined in healthy individuals. This paper brings unique results on the application of ultra-sensitive electroanalytical method for metallothionein detection. The detection limit and other analyti...Continue Reading

References

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Citations

Jul 14, 2012·Metallomics : Integrated Biometal Science·Petr BabulaRene Kizek
Oct 12, 2015·Journal of Pharmaceutical and Biomedical Analysis·Sona KrizkovaVojtech Adam
Sep 8, 2011·Journal of Separation Science·Marta ZalewskaHalina Milnerowicz
Mar 15, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xilin XiaoBo He
Sep 27, 2013·Metallomics : Integrated Biometal Science·Martina RaudenskaMichal Masarik
Nov 28, 2012·PloS One·Pavlina SobrovaRene Kizek
Mar 14, 2017·International Journal of Molecular Sciences·Miguel Angel Merlos RodrigoVojtech Adam
Jun 8, 2013·International Journal of Molecular Sciences·Jarmila KruseovaTomas Eckschlager
Jun 3, 2015·Electrophoresis·Lukas NejdlRene Kizek
Oct 17, 2015·PloS One·Yulia A ZatulovskaiaLudmila V Puchkova
May 18, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Michal MasarikRene Kizek
Oct 15, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xilin XiaoYimou Wu

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

GPES
Biolight

Related Concepts